Suda Yoshihito, Sasaki Nana, Kagawa Kyoma, Elean Mariano, Zhou Binghui, Tomokiyo Mikado, Islam Md Aminul, Rajoka Muhammad Shahid Riaz, Kober A K M Humayun, Shimazu Tomoyuki, Egusa Shintaro, Terashima Yuji, Aso Hisashi, Ikeda-Ohtsubo Wakako, Villena Julio, Kitazawa Haruki
Department of Food Resource Development, School of Food Industrial Sciences, Miyagi University, Sendai 982-0215, Japan.
Graduate School of Food, Agricultural and Environmental Sciences, Miyagi University, Sendai 982-0215, Japan.
Microorganisms. 2021 Apr 25;9(5):921. doi: 10.3390/microorganisms9050921.
subsp. TUA4408L is able to differentially modulate the innate immune response of porcine intestinal epithelial cells triggered by TLR4 activation. This strain also has a remarkable ability to grow on plant substrates. These two immunological and biotechnological characteristics prompted us to evaluate whether the soymilk by-product okara fermented with the TUA4408L strain can serve as an immunobiotic feed with the ability to beneficially modulate the intestinal immunity of piglets after weaning to improve their productivity. Our in vivo studies demonstrated that the administration of immunobiotic TUA4408L-fermented okara feed significantly increased piglet growth performance and meat quality. These positive effects were associated with the ability of the TUA4408L-fermented okara feed to beneficially modulate both intestinal microbiota and immunity in pigs. The immunobiotic feed improved the abundance of the beneficial bacteria and in the gut of pigs, reduced blood markers of inflammation, and differentially regulated the expression of inflammatory and regulatory cytokines in the intestinal mucosa. These findings indicate that the immunobiotic TUA4408L-fermented okara feed could be an economical and environmentally friendly option to improve the growth performance and immune health of pigs.
亚种TUA4408L能够差异性地调节由TLR4激活引发的猪肠道上皮细胞的先天免疫反应。该菌株还具有在植物底物上生长的显著能力。这两种免疫学和生物技术特性促使我们评估用TUA4408L菌株发酵的豆浆副产物豆渣是否可以作为一种免疫生物饲料，具有有益调节断奶仔猪肠道免疫力以提高其生产性能的能力。我们的体内研究表明，给予免疫生物TUA4408L发酵豆渣饲料显著提高了仔猪的生长性能和肉质。这些积极作用与TUA4408L发酵豆渣饲料有益调节猪的肠道微生物群和免疫力的能力有关。这种免疫生物饲料提高了猪肠道中有益细菌的丰度，降低了血液炎症标志物，并差异性地调节了肠道黏膜中炎症和调节性细胞因子的表达。这些发现表明，免疫生物TUA4408L发酵豆渣饲料可能是提高猪生长性能和免疫健康的一种经济且环保的选择。
