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A rapid method for processing very large numbers of tissue sections for immunohistochemical hybridoma screening.

作者信息

Marusich M F

机构信息

Department of Biology, University of Oregon, Eugene 97403.

出版信息

J Immunol Methods. 1988 Jun 28;111(1):143-5. doi: 10.1016/0022-1759(88)90071-3.

DOI:10.1016/0022-1759(88)90071-3
PMID:3392396
Abstract

A hybridoma screening format which facilitates the processing of thousands of tissue sections for immunohistochemical analysis is described. The method utilizes sterile replica transfers of monoclonal antibody-containing test supernatants from 96-well culture plates to tissue sections mounted in appropriately sized 8 X 12 arrays, and is extremely rapid and inexpensive.

摘要

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