Furst A, Mahowald A P
J Immunol Methods. 1984 May 11;70(1):101-9. doi: 10.1016/0022-1759(84)90394-6.
We describe a rapid procedure for immunofluorescent screening of hybridoma supernatants. Use of primary cell cultures as substrates allows immediate detection and partial characterization of antibodies binding selectively to specific cell types. Sulfonated tissue culture cluster lids are used as a single substrate upon which cells are cultured and all stages of the antibody binding assay are performed, including microscopic observation. They may also be used for mounting cryostat sections of tissues. Condensation rings on the lids isolate individual groups of cells for each assay. Cells may be live or fixed, as desired. Very small volumes of culture supernatant are required for each assay, and nearly all steps are performed in bulk.
我们描述了一种用于杂交瘤上清液免疫荧光筛选的快速方法。使用原代细胞培养物作为底物可立即检测并部分鉴定选择性结合特定细胞类型的抗体。磺化组织培养板盖用作单个底物，在其上培养细胞并进行抗体结合测定的所有阶段，包括显微镜观察。它们还可用于固定组织切片的封片。盖子上的冷凝环可为每次测定分离单个细胞组。细胞可根据需要为活细胞或固定细胞。每次测定所需的培养上清液体积非常小，几乎所有步骤都可批量进行。
