Cohn J S, McNamara J R, Cohn S D, Ordovas J M, Schaefer E J
USDA Human Nutrition Research Center on Aging, Tufts University, Boston, MA 02111.
J Lipid Res. 1988 Apr;29(4):469-79.
Plasma lipoprotein changes were monitored for 12 hr after a fat-rich meal (1 g of fat/kg body weight) in 22 subjects (9 males, 13 females, 22-79 yr old). Plasma triglyceride, measured hourly, peaked once in some subjects, but twice or three times in others. The magnitude of postprandial triglyceridemia varied considerably between subjects (range: 650-4082 mg.hr/dl). Males tended to have greater postprandial triglyceridemia than females, and elderly subjects had significantly (P less than 0.05) greater postprandial triglyceridemia than younger subjects. Total plasma cholesterol, measured every three hr, increased significantly (6.0 +/- 2.1%) in 7 subjects, decreased significantly (7.1 +/- 1.2%) in 10 subjects, and remained unchanged in the remainder. Single spin ultracentrifugation and dextran sulfate precipitation procedures were used to quantitate triglyceride and cholesterol in triglyceride-rich lipoproteins (TRL, d less than 1.006 g/ml), low density lipoproteins (LDL), and high density lipoproteins (HDL). Plasma TRL and HDL triglyceride increased after the fat meal, while LDL triglyceride decreased at 3 hr but increased at 9 and 12 hr. TRL cholesterol increased postprandially, while LDL and HDL cholesterol decreased. Phospholipid (PL), free (FC) and esterified (EC) cholesterol measurements were carried out on the plasma and lipoprotein fractions of 8 subjects. Plasma PL increased significantly at 3, 6, and 9 hr after the fat-rich meal, due to increases in TRL and HDL PL. TRL CE increased postprandially, but a greater decrease in LDL and HDL CE caused plasma CE to be decreased. Plasma FC increased, predominantly due to an increase in TRL FC. Plasma concentrations of apolipoprotein A-I and apolipoprotein B both decreased after the fat-rich meal. The magnitude of postprandial triglyceridemia was inversely correlated with HDL cholesterol levels (r = -0.502, P less than 0.05) and positively correlated with age (r = -0.449, P less than 0.05), fasting levels of plasma triglyceride (r = 0.636, P less than 0.01), plasma apoB (r = 0.510, P less than 0.05), TRL triglyceride (r = 0.564, P less than 0.01), TRL cholesterol (r = 0.480, P less than 0.05) and LDL triglyceride (r = 0.566, P less than 0.01). Change in postprandial cholesterolemia was inversely correlated with fasting levels of HDL cholesterol (r = -0.451, P less than 0.05) and plasma apoA-I (r = -0.436, P less than 0.05).(ABSTRACT TRUNCATED AT 400 WORDS)
在22名受试者（9名男性，13名女性，年龄22 - 79岁）中，进食富含脂肪的餐食（1克脂肪/千克体重）后监测血浆脂蛋白变化12小时。每小时测量血浆甘油三酯，部分受试者出现一次峰值，而其他受试者出现两次或三次峰值。餐后甘油三酯血症的程度在受试者之间差异很大（范围：650 - 4082毫克·小时/分升）。男性的餐后甘油三酯血症往往比女性更严重，老年受试者的餐后甘油三酯血症比年轻受试者显著更高（P < 0.05）。每三小时测量一次总血浆胆固醇，7名受试者显著升高（6.0±2.1%），10名受试者显著降低（7.1±1.2%），其余受试者保持不变。采用单旋转超速离心和硫酸葡聚糖沉淀法对富含甘油三酯的脂蛋白（TRL，密度<1.006克/毫升）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）中的甘油三酯和胆固醇进行定量。脂肪餐后血浆TRL和HDL甘油三酯升高，而LDL甘油三酯在3小时降低，但在9小时和12小时升高。TRL胆固醇餐后升高，而LDL和HDL胆固醇降低。对8名受试者的血浆和脂蛋白组分进行磷脂（PL）、游离胆固醇（FC）和酯化胆固醇（EC）测量。富含脂肪的餐食后3、6和9小时血浆PL显著升高，这是由于TRL和HDL的PL增加。TRL CE餐后升高，但LDL和HDL CE的更大降低导致血浆CE降低。血浆FC升高，主要是由于TRL FC增加。富含脂肪的餐食后载脂蛋白A - I和载脂蛋白B的血浆浓度均降低。餐后甘油三酯血症的程度与HDL胆固醇水平呈负相关（r = -0.502，P < 0.05），与年龄呈正相关（r = -0.449，P < 0.05），与空腹血浆甘油三酯水平（r = 0.636，P < 0.01）、血浆载脂蛋白B（r = 0.510，P < 0.05）、TRL甘油三酯（r = 0.564，P < 0.01）、TRL胆固醇（r = 0.480，P < 0.05）和LDL甘油三酯（r = 0.566，P < 0.01）呈正相关。餐后胆固醇血症的变化与空腹HDL胆固醇水平（r = -0.451，P < 0.05）和血浆载脂蛋白A - I（r = -0.436，P < < 0.05）呈负相关。（摘要截断于400字）
