Martiniakova Monika, Bobonova Ivana, Toman Robert, Galik Branislav, Bauerova Maria, Omelka Radoslav
Department of Zoology and Anthropology, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, 949 74 Nitra, Slovakia.
Department of Botany and Genetics, Faculty of Natural Sciences, Constantine the Philosopher University in Nitra, 949 74 Nitra, Slovakia.
Animals (Basel). 2021 Apr 28;11(5):1265. doi: 10.3390/ani11051265.
Bee pollen has been successfully used as a feed additive with beneficial impacts on productive, reproductive, and immune conditions of animals. However, its effect on bone structure and bone health remains controversial. Therefore, the purpose of our study was to examine the impact of bee pollen supplementation on macroscopic and microscopic structure of a femoral bone using rats as suitable animal models. Male rats (1 month-old) were assigned into three groups: control (C group) that was fed a standard diet without bee pollen and two bee pollen supplemented groups (P1 and P2 groups) that received an experimental diet including 0.5% and 0.75% of bee pollen, respectively, for 3 months. A number of unfavorable effects of 0.75% bee pollen administration on bone weight, cortical bone thickness, calcium content, alkaline phosphatase activity, sizes of primary osteons' vascular canals, Haversian canals and secondary osteons in the cortical bone have been recorded, whereas these bone parameters were significantly decreased in the P2 group versus the C group. On the contrary, the concentration of 0.5% did not affect any of bone features mentioned above. In conclusion, the impact of bee pollen supplementation on femoral bone structure of rats depends on the dose used.
蜂花粉已成功用作饲料添加剂，对动物的生产性能、繁殖能力和免疫状况产生有益影响。然而，其对骨骼结构和骨骼健康的影响仍存在争议。因此，我们研究的目的是使用大鼠作为合适的动物模型，研究补充蜂花粉对股骨宏观和微观结构的影响。将1月龄雄性大鼠分为三组：对照组（C组）喂食不含蜂花粉的标准饮食，两个补充蜂花粉的组（P1组和P2组）分别接受含0.5%和0.75%蜂花粉的实验饮食，持续3个月。记录到给予0.75%蜂花粉对骨重量、皮质骨厚度、钙含量、碱性磷酸酶活性、皮质骨中初级骨单位血管通道、哈弗斯管和次级骨单位大小产生了一些不利影响，而P2组与C组相比，这些骨骼参数显著降低。相反，0.5%的浓度对上述任何骨骼特征均无影响。总之，补充蜂花粉对大鼠股骨结构的影响取决于所使用的剂量。
