Taghipour Simin, Abastabar Mahdi, Piri Fahimeh, Aboualigalehdari Elham, Jabbari Mohammad Reza, Zarrinfar Hossein, Nouripour-Sisakht Sadegh, Mohammadi Rasoul, Ahmadi Bahram, Ansari Saham, Katiraee Farzad, Niknejad Farhad, Didehdar Mojtaba, Nazeri Mehdi, Makimura Koichi, Rezaei-Matehkolaei Ali
Department of Medical Parasitology and Mycology, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord 88157-13471, Iran.
Invasive Fungi Research Center, Department of Medical Mycology and Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari 48157-33971, Iran.
J Fungi (Basel). 2021 Apr 28;7(5):345. doi: 10.3390/jof7050345.
A molecular epidemiology study was conducted between 2016 and 2017 by a network of collaborators from 12 provinces in the Islamic Republic of Iran. A total of 1484 soil samples from different habitats were screened for the presence of dermatophytes by using the hair baiting technique. The primary identification of isolates was carried out by amplification and I restriction fragment length polymorphism (RFLP) of the internal transcribed spacers regions of ribosomal DNA (ITS-rDNA). The identifications, especially in the cases of isolates with unknown RFLP patterns, were confirmed by sequencing of the ITS-rDNA region. As a result, 256 isolates were recovered. The isolation rate was higher in soils with pH range 7.1-8.0, collected from animal habitats ( = 78; 34%) and parks and gardens ( = 75; 32%), geographically from Mazandaran Province ( = 115; 49.5%) and seasonally in the spring ( = 129; 50.4%), all of which were statistically significant ( < 0.05). The dermatophytes comprising five species of the two genera, viz., ( = 214), ( = 34), ( = 5), ( = 2) and ( = 1), were isolated. The geophilic dermatophytes occurred in various soils from different parts of Iran; however, surprisingly, emerged as the dominant species, outnumbering the common geophilic species of . For the definitive identification of soil inhabitant dermatophytes, DNA-based identification is strongly recommended.
2016年至2017年期间，来自伊朗伊斯兰共和国12个省份的合作网络开展了一项分子流行病学研究。通过毛发诱饵技术，对来自不同栖息地的1484份土壤样本进行了皮肤癣菌筛查。通过对核糖体DNA（ITS-rDNA）内部转录间隔区进行扩增和I型限制性片段长度多态性（RFLP）分析，对分离株进行初步鉴定。对于RFLP模式未知的分离株，通过对ITS-rDNA区域进行测序来确认鉴定结果。结果，共分离出256株分离株。在pH值为7.1 - 8.0的土壤中，从动物栖息地（n = 78；34%）和公园及花园（n = 75；32%）采集的样本中，分离率较高，从地理位置上看来自马赞德兰省（n = 115；49.5%），从季节上看在春季（n = 129；50.4%），所有这些差异均具有统计学意义（P < 0.05）。分离出的皮肤癣菌包括两个属的五个种，即，毛癣菌属（n = 214）、小孢子菌属（n = 34）、表皮癣菌属（n = 5）、镰刀菌属（n = 2）和柱顶孢属（n = 1）。嗜土性皮肤癣菌存在于伊朗不同地区的各种土壤中；然而，令人惊讶的是，红色毛癣菌成为优势种，数量超过了常见的嗜土性须癣毛癣菌。对于土壤中栖息的皮肤癣菌的最终鉴定，强烈推荐基于DNA的鉴定方法。
