Dehghan Parvin, Yousefi Jalali Shabaz, Chadeganipour Mostafa
Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2019 Jun 28;8:38. doi: 10.4103/abr.abr_31_19. eCollection 2019.
Dermatophytes are one of the most important etiologic agents of cutaneous infections in humans and animals. The present study aimed to study the frequency distribution of keratinophilic dermatophyte fungi using conventional and molecular methods in soil of Isfahan city.
In this study, 200 soil samples were randomly selected in three northern, southern, and central parts of Isfahan using hair-baiting technique. The fungi were identified by morphology based on macroscopic and microscopic characteristics of fungi. Furthermore, the sequencing of ITS1-5.8S-ITS2 region of the ribosomal DNA of the 60 randomly isolated fungi was investigated.
The results of conventional method showed that from a total of 371 fungal colonies, the highest amount of detected colonies was in the central zone (151, 40.26%). Furthermore, in all three areas, the most common detected dermatophyte was (38.3%). The results of the molecular analysis showed that identified by the morphology method was , and also, sp. with the frequency of 30% in morphology method was the second dominated fungus including (42.6%) and (21.4%) which confirmed by sequencing method.
The results showed that keratinophilic dermatophyte fungi including , and are found in the children playgrounds in Isfahan. Therefore, health-care officials should pay more attention to these hygienic issues. , which is found here for the first time in Iran, has been reported only from China.
皮肤癣菌是人和动物皮肤感染最重要的病原体之一。本研究旨在采用传统方法和分子方法研究伊斯法罕市土壤中嗜角质皮肤癣菌的频率分布。
在本研究中，采用毛发诱饵技术在伊斯法罕市北部、南部和中部三个区域随机选取200份土壤样本。根据真菌的宏观和微观特征，通过形态学鉴定真菌。此外，还对随机分离出的60株真菌的核糖体DNA的ITS1-5.8S-ITS2区域进行了测序。
传统方法的结果显示，在总共371个真菌菌落中，检测到的菌落数量最多的是中部区域（151个，40.26%）。此外，在所有三个区域中，最常见的检测到的皮肤癣菌是 （38.3%）。分子分析结果表明，形态学方法鉴定出的 为 ，并且，形态学方法中频率为30%的 属真菌是第二大优势真菌，包括 （42.6%）和 （21.4%），这通过测序方法得到了证实。
结果表明，伊斯法罕市的儿童游乐场中存在嗜角质皮肤癣菌，包括 、 和 。因此，卫生保健官员应更加关注这些卫生问题。 在伊朗首次在此发现，此前仅在中国有报道。
