Moulíková Š, Kolařík M, Lorch J M, Kolarczyková D, Hubka V, Čmoková A
Department of Botany, Faculty of Science, Charles University, Prague, Czech Republic.
Laboratory of Fungal Genetics and Metabolism, Institute of Microbiology of the Czech Academy of Sciences, Prague, Czech Republic.
Persoonia. 2023 Jun;50:27-47. doi: 10.3767/persoonia.2023.50.02. Epub 2023 Feb 1.
is the most diverse genus of dermatophytes, and its natural reservoir is considered to be soil enriched by keratin sources. During a study on the diversity of dermatophytes in wild small rodents in the Czech Republic, we isolated several strains of . To explore the diversity and ecological significance of these isolates from rodents (n = 29), we characterised the strains genetically (i.e., sequenced ITS, and ), morphologically, physiologically, and by conducting mating experiments. We then compared the rodent-derived strains to existing ITS sequence data from GenBank and the GlobalFungi Database to further investigate biogeography and the association of species with different types of environments. In total, eight species were isolated from rodents, including four previously described species (, , , ) and four new species proposed herein, i.e., , , and . The geographical distribution of these newly described species was not restricted to the Czech Republic nor rodents. Additional isolates were obtained from bats and other mammals, reptiles, and soil from Europe, North America, and Asia. Data mining showed that the genus has a diverse ecology, with some lineages occurring relatively frequently in soil, whereas others appeared to be more closely associated with live animals, as we observed in . Low numbers of sequence reads ascribed to in soil show that the genus is rare in this environment, which supports the hypothesis that spp. are not soil generalists but rather strongly associated with animals and keratin debris. This is the first study to utilise existing metabarcoding data to assess biogeographical, ecological, and diversity patterns in dermatophytes. : Moulíková Š, Kolařík M, Lorch JM, et al. 2022. Wild rodents harbour high diversity of Arthroderma. Persoonia 50: 27- 47. https://doi.org/10.3767/persoonia.2023.50.02.
是皮肤癣菌中最多样化的属，其天然宿主被认为是富含角蛋白来源的土壤。在对捷克共和国野生小型啮齿动物皮肤癣菌多样性的研究中，我们分离出了几种 菌株。为了探究这些来自啮齿动物的分离株（n = 29）的多样性和生态意义，我们对这些菌株进行了基因特征分析（即对ITS、 和 进行测序）、形态学分析、生理学分析，并进行了交配实验。然后，我们将来自啮齿动物的菌株与GenBank和全球真菌数据库中现有的ITS序列数据进行比较，以进一步研究生物地理学以及 物种与不同类型环境的关联。总共从啮齿动物中分离出了8种 物种，包括4种先前描述的物种（ 、 、 、 ）以及本文提出的4个新物种，即 、 、 和 。这些新描述物种的地理分布并不局限于捷克共和国，也不局限于啮齿动物。还从蝙蝠和其他哺乳动物、爬行动物以及来自欧洲、北美和亚洲的土壤中获得了其他分离株。数据挖掘表明，该属具有多样的生态学特征，一些谱系在土壤中相对频繁出现，而其他谱系似乎与活体动物关系更密切，正如我们在 中观察到的那样。土壤中归属于 的序列读数较少，表明该属在这种环境中很罕见，这支持了 物种不是土壤泛生菌而是与动物和角蛋白碎片密切相关的假设。这是第一项利用现有宏条形码数据评估皮肤癣菌生物地理学、生态学和多样性模式的研究。：Moulíková Š，Kolařík M，Lorch JM等。2022年。野生啮齿动物中存在高度多样的节皮菌属。《真菌学报》50：27 - 47。https://doi.org/10.3767/persoonia.2023.50.02 。
