State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Life Science and Technology, Guangxi University, Nanning 530004, China.
Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Sciences, South China Normal University, Guangzhou 510631, China.
Genes (Basel). 2021 Apr 27;12(5):656. doi: 10.3390/genes12050656.
The Mediator complex transduces information from the DNA-bound transcription factors to the RNA polymerase II transcriptional machinery. Research on plant Mediator subunits has primarily been performed in Arabidopsis, while very few of them have been functionally characterized in rice. In this study, the rice Mediator subunit 16, was examined. encodes a putative protein of 1301 amino acids, which is longer than the version previously reported. It was expressed in various rice organs and localized to the nucleus. The knockout of resulted in rice seedling lethality. Its overexpression led to the retardation of rice growth, low yield, and spontaneous cell death in the leaf blade and sheath. RNA sequencing suggested that the overexpression of altered the expression of a large number of genes. Among them, the upregulation of some defense-related genes was verified. can regulate the expression of a wealth of genes, and alterations in its expression have a profound impact on plant growth, development, and defense responses in rice.
中介复合物将 DNA 结合转录因子的信息传递给 RNA 聚合酶 II 转录机制。植物中介亚基的研究主要在拟南芥中进行，而在水稻中，只有很少的亚基具有功能特征。在这项研究中，研究了水稻中介亚基 16。编码一个推测的 1301 个氨基酸的蛋白质，比以前报道的版本更长。它在各种水稻器官中表达，并定位于细胞核。的敲除导致水稻幼苗致死。其过表达导致水稻生长迟缓、产量低以及叶片和叶鞘自发细胞死亡。RNA 测序表明，过表达改变了大量基因的表达。其中，一些与防御相关基因的上调得到了验证。可以调节大量基因的表达，其表达的改变对水稻的生长、发育和防御反应有深远的影响。
