Selçuk University, Department of Chemistry, 42150, Konya, Turkey.
Konya Technical University, Department of Chemical Engineering, 42150, Konya, Turkey.
Anal Chim Acta. 2021 Jun 1;1162:338494. doi: 10.1016/j.aca.2021.338494. Epub 2021 Apr 9.
It is crucial to determine and control the metronidazole (MET) ingredient in food and pharmaceuticals for human health and food safety. Even though many sensors have been previously reported to detect MET, there is still a need for a highly selective and sensitive, easy, fast, cost-effective sensor in this area. Herein, we report a fluorescent calix[4]arene derivative (PIMC) for highly selective and sensitive and facile and rapid MET detection based on fluorescence (FL) quenching. The highest FL quenching occurs when PIMC is exposed to MET solution at 400 nm (λ = 340). Owing to the quenching efficacy of MET linearly up to 5.5 × 10 nM was obtained a detection limit of 2.44 nM. Besides, interferences of other pharmaceuticals and ions on probe performance were investigated. The FL probe was successful in MET detection without the assistance of any separation techniques in a pharmaceutical sample (tablet) with an acceptable recovery of 101.3%. The applicability of the current probe as a paper-based sensor to MET detection has been successfully tested. As a result, the proposed probe presents a fast and suitable strategy to sensitive and selective detect MET and proves a good potential for practical applications, especially pharmaceutical preparations.
确定和控制食品和药品中的甲硝唑（MET）成分对于人类健康和食品安全至关重要。尽管以前已经有许多传感器被报道用于检测 MET，但在该领域仍然需要一种高度选择性和灵敏、简单、快速、具有成本效益的传感器。在此，我们报告了一种基于荧光（FL）猝灭的高选择性和灵敏、简单和快速的 MET 检测用荧光杯[4]芳烃衍生物（PIMC）。当 PIMC 暴露于 MET 溶液中时，在 400nm（λ=340）处发生最高的 FL 猝灭。由于 MET 的猝灭效果呈线性关系，直至 5.5×10 nM，检测限为 2.44 nM。此外，还研究了其他药物和离子对探针性能的干扰。在没有任何分离技术辅助的情况下，FL 探针成功地在药物样品（片剂）中检测到 MET，回收率可接受，为 101.3%。当前探针作为一种用于 MET 检测的基于纸张的传感器的适用性已成功得到测试。因此，所提出的探针为敏感和选择性检测 MET 提供了一种快速且合适的策略，并证明了其在实际应用中的良好潜力，特别是在药物制剂中。
