Department of Clinical Laboratory Medicine, The Third Affiliated Hospital of Guangzhou Medical University, Guangzhou 510150, China.
Department of Clinical Medicine, The Third Clinical School of Guangzhou Medical University, Guangzhou 511436, China.
Biomed Res Int. 2021 Apr 8;2021:6614812. doi: 10.1155/2021/6614812. eCollection 2021.
High mortality associated with carbapenemase-producing Gram-negative bacteria (CP-GNB) has evolved into a global health threat. Rapid and accurate detection as well as prompt treatment are of great significance in this case. Xpert Carba-R, a multiple qualitative analysis designed to detect five clinically relevant carbapenem-resistant gene families within one hour, is regarded as reliable, accurate, and easy-to-operate. This study is to present a systematic evaluation of the performance of Xpert Carba-R in detecting carbapenemase genes in GNB suspected for carbapenemase production.
We searched and screened the literature on "Xpert Carba-R" in the database of PubMed, Web of Science, Embase, and Cochrane Library, employing two independent evaluators to collect data, respectively. Then, statistical analysis of the data obtained was performed by the Stata 12.0 software to measure the accuracy of Xpert Carba-R assay in detecting the carbapenemase genes in GNB.
We screened a total of 1767 Gram-negative bacillus isolates documented in 9 articles. The precision of the detection of OXA-48 carbapenemase genes was 100%; that of NDM = 100%; that of VIM = 100%. When it came to KPC, the precision rate was 100%; that of IMP = 99%. The overall accuracy of the detection of carbapenemase genes was 100%.
Xpert Carba-R assay demonstrates a 100% precision in identifying carbapenemase genes in GNB. It can be seen that Xpert Carba-R method is an effective tool for early clinical detection, which is suitable for the detection of carbapenase gene in GNB.
产碳青霉烯酶革兰氏阴性菌（CP-GNB）死亡率高，已成为全球健康威胁。快速准确的检测和及时治疗对此非常重要。Xpert Carba-R 是一种多质分析，旨在在一小时内检测到五种临床相关的碳青霉烯耐药基因家族，被认为是可靠、准确和易于操作的。本研究旨在对 Xpert Carba-R 检测革兰氏阴性菌中产碳青霉烯酶基因的性能进行系统评价。
我们在 PubMed、Web of Science、Embase 和 Cochrane Library 数据库中搜索并筛选了“Xpert Carba-R”的文献，由两名独立评估员分别收集数据。然后，使用 Stata 12.0 软件对获得的数据进行统计分析，以测量 Xpert Carba-R 检测革兰氏阴性菌中产碳青霉烯酶基因的准确性。
我们共筛选了 9 篇文献中记录的 1767 株革兰氏阴性杆菌分离株。OXA-48 碳青霉烯酶基因检测的精确率为 100%；NDM=100%；VIM=100%。对于 KPC，准确率为 100%；IMP=99%。碳青霉烯酶基因检测的总准确率为 100%。
Xpert Carba-R 检测法在鉴定革兰氏阴性菌中的碳青霉烯酶基因方面具有 100%的精度。由此可见，Xpert Carba-R 方法是一种早期临床检测的有效工具，适用于革兰氏阴性菌中碳青霉烯酶基因的检测。
