Flow Cytometry Diagnostic Center and Immunotherapy (CDCI), AOU Careggi, Florence, Italy.
Methods Mol Biol. 2021;2285:99-109. doi: 10.1007/978-1-0716-1311-5_8.
T-cell receptor (TCR)-Vβ repertoire analysis is a sensitive method for detection of T-cell clonality. This type of analysis has been used for studying selective T-cell responses in autoimmune disease, alloreactivity in transplantation, and protective immunity against microbial and tumor antigens and in neoplastic T cells. Here, we describe the flow cytometric methods to perform this analysis.
T 细胞受体（TCR）-Vβ 谱分析是一种用于检测 T 细胞克隆性的敏感方法。这种分析方法已用于研究自身免疫性疾病中的选择性 T 细胞反应、移植中的同种反应、针对微生物和肿瘤抗原的保护性免疫以及肿瘤性 T 细胞。在这里，我们描述了进行这种分析的流式细胞术方法。
