Department of Medical Genetics/Prenatal Diagnostic Center, West China Second University Hospital, Sichuan University, Chengdu, China.
Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, China.
Cancer Med. 2024 Sep;13(17):e70164. doi: 10.1002/cam4.70164.
T cells, the "superstar" of the immune system, play a crucial role in antitumor immunity. T-cell receptors (TCR) are crucial molecules that enable T cells to identify antigens and start immunological responses. The body has evolved a unique method for rearrangement, resulting in a vast diversity of TCR repertoires. A healthy TCR repertoire is essential for the particular identification of antigens by T cells.
In this article, we systematically summarized the TCR creation mechanisms and analysis methodologies, particularly focusing on the application of next-generation sequencing (NGS) technology. We explore the TCR repertoire in health and cancer, and discuss the implications of TCR repertoire analysis in understanding carcinogenesis, cancer progression, and treatment.
The TCR repertoire analysis has enormous potential for monitoring the emergence and progression of malignancies, as well as assessing therapy response and prognosis. The application of NGS has dramatically accelerated our comprehension of TCR diversity and its role in cancer immunity.
To substantiate the significance of TCR repertoires as biomarkers, more thorough and exhaustive research should be conducted. The TCR repertoire analysis, enabled by advanced sequencing technologies, is poised to become a crucial tool in the future of cancer diagnosis, monitoring, and therapy evaluation.
T 细胞是免疫系统的“超级明星”，在抗肿瘤免疫中起着至关重要的作用。T 细胞受体（TCR）是使 T 细胞能够识别抗原并启动免疫反应的关键分子。机体进化出一种独特的重排方式，从而产生了大量多样性的 TCR 库。健康的 TCR 库对于 T 细胞对特定抗原的识别至关重要。
本文系统总结了 TCR 的产生机制和分析方法，特别关注下一代测序（NGS）技术的应用。我们探讨了 TCR 库在健康和癌症中的作用，并讨论了 TCR 库分析在理解致癌作用、癌症进展和治疗中的意义。
TCR 库分析在监测恶性肿瘤的发生和进展、评估治疗反应和预后方面具有巨大的潜力。NGS 的应用极大地促进了我们对 TCR 多样性及其在癌症免疫中的作用的理解。
为了证实 TCR 库作为生物标志物的意义，需要进行更彻底和详尽的研究。先进的测序技术使 TCR 库分析有望成为癌症诊断、监测和治疗评估未来的重要工具。
