National Heart and Lung Institute, Imperial College London, London, UK.
Methods Mol Biol. 2021;2285:329-348. doi: 10.1007/978-1-0716-1311-5_25.
Asthma is a highly prevalent lung disease, characterized by airway dysfunction and chronic inflammation. Asthma occurs in both children and adults, but frequently originates in early life. Heterogeneous asthma phenotypes exist, but Th2 cells are key players in a large proportion of cases, while other CD4+ T cell subsets are also implicated in driving and limiting pathology. In this chapter, we describe methods for establishing allergic airway disease to model asthma in adult and neonatal mice, along with protocols for measuring key disease parameters and quantifying and phenotyping CD4+ T cell subtypes.
哮喘是一种高发肺部疾病，其特征为气道功能障碍和慢性炎症。哮喘可发生于儿童和成人，但通常在生命早期就已出现。哮喘存在多种表型，但 Th2 细胞是大多数情况下的关键致病因素，其他 CD4+T 细胞亚群也参与了疾病的发生和发展。在本章中，我们将描述在成年和新生小鼠中建立变应性气道疾病模型来模拟哮喘的方法，以及测量关键疾病参数、定量和表型分析 CD4+T 细胞亚群的方案。
