• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

晚期胃癌患者循环游离 DNA 中 RASSF1A、SOX17 和 Wif-1 启动子甲基化状态的预后作用。

Prognostic Role of RASSF1A, SOX17 and Wif-1 Promoter Methylation Status in Cell-Free DNA of Advanced Gastric Cancer Patients.

机构信息

Department of Oncology, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.

Department of Medical Statistics, Medical School, Democritus University of Thrace, Alexandroupolis, Greece.

出版信息

Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033820973279. doi: 10.1177/1533033820973279.

DOI:10.1177/1533033820973279
PMID:33928818
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8113658/
Abstract

Epigenetic modification of several genes is a key component in the development of gastric cancer. The methylation status of , and genes was evaluated in the cell free circulating DNA of 70 patients with advanced gastric cancer, using methylation-specific PCR. Patients with higher cell-free DNA concentration seem to have lower PFS, than patients with lower cell-free DNA concentration (p = 0.001). was the tumor suppressor gene, most frequently methylated in metastatic gastric cancer patients, followed by and (74.3%, 60.0% and 47.1%, respectively). Patients having the promoter methylated, had lower progression free survival and overall survival, than unmethylated ones (p < 0.001). Patients having the promoter methylated, had lower progression free survival and overall survival, than unmethylated ones (p = 0.001). Patients having the promoter methylated, had lower progression free survival and overall survival, than unmethylated ones (p = 0.004). Promoter methylation of the examined genes was significantly associated with a decrease in progression free survival and overall survival, comparing to that of patients without methylation. Simultaneous methylation of the above genes was associated with even worse progression free survival and overall survival. The methylation of , and and genes, is a frequent epigenetic event in patients with advanced gastric cancer.

摘要

基因的表观遗传修饰是胃癌发展的一个关键组成部分。使用甲基化特异性 PCR 评估了 70 例晚期胃癌患者的无细胞循环 DNA 中 、 和 基因的甲基化状态。细胞游离 DNA 浓度较高的患者似乎比细胞游离 DNA 浓度较低的患者 PFS 更差（p=0.001）。 是肿瘤抑制基因，在转移性胃癌患者中最常发生甲基化，其次是 和 （分别为 74.3%、60.0%和 47.1%）。与未甲基化的患者相比， 启动子甲基化的患者无进展生存期和总生存期较低（p<0.001）。与未甲基化的患者相比， 启动子甲基化的患者无进展生存期和总生存期较低（p=0.001）。与未甲基化的患者相比， 启动子甲基化的患者无进展生存期和总生存期较低（p=0.004）。与未甲基化的患者相比，与不甲基化的患者相比，上述基因启动子甲基化与无进展生存期和总生存期的降低显著相关。上述基因的同时甲基化与更差的无进展生存期和总生存期相关。 、 和 基因的甲基化是晚期胃癌患者中常见的表观遗传事件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/2f2cbdd90657/10.1177_1533033820973279-fig14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/e71a0cfc7b59/10.1177_1533033820973279-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/189ebdef30bc/10.1177_1533033820973279-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/a4623f1cdd62/10.1177_1533033820973279-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/230cce836b5f/10.1177_1533033820973279-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/36eefdc5e02f/10.1177_1533033820973279-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/77df2e927029/10.1177_1533033820973279-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/2e4aa9e4e0c1/10.1177_1533033820973279-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/41a731e9a495/10.1177_1533033820973279-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/52824596d5ae/10.1177_1533033820973279-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/b071e92d402c/10.1177_1533033820973279-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/271cf57774d7/10.1177_1533033820973279-fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/b89965fdbea2/10.1177_1533033820973279-fig12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/5b8887c9a137/10.1177_1533033820973279-fig13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/2f2cbdd90657/10.1177_1533033820973279-fig14.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/e71a0cfc7b59/10.1177_1533033820973279-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/189ebdef30bc/10.1177_1533033820973279-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/a4623f1cdd62/10.1177_1533033820973279-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/230cce836b5f/10.1177_1533033820973279-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/36eefdc5e02f/10.1177_1533033820973279-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/77df2e927029/10.1177_1533033820973279-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/2e4aa9e4e0c1/10.1177_1533033820973279-fig7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/41a731e9a495/10.1177_1533033820973279-fig8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/52824596d5ae/10.1177_1533033820973279-fig9.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/b071e92d402c/10.1177_1533033820973279-fig10.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/271cf57774d7/10.1177_1533033820973279-fig11.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/b89965fdbea2/10.1177_1533033820973279-fig12.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/5b8887c9a137/10.1177_1533033820973279-fig13.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7203/8113658/2f2cbdd90657/10.1177_1533033820973279-fig14.jpg

相似文献

1
Prognostic Role of RASSF1A, SOX17 and Wif-1 Promoter Methylation Status in Cell-Free DNA of Advanced Gastric Cancer Patients.晚期胃癌患者循环游离 DNA 中 RASSF1A、SOX17 和 Wif-1 启动子甲基化状态的预后作用。
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033820973279. doi: 10.1177/1533033820973279.
2
Association between SOX17, Wif-1 and RASSF1A promoter methylation status and response to chemotherapy in patients with metastatic gastric cancer.SOX17、Wif-1和RASSF1A启动子甲基化状态与转移性胃癌患者化疗反应之间的关联。
Clin Chem Lab Med. 2020 Sep 1;59(2):e73-e75. doi: 10.1515/cclm-2020-0662.
3
Prognostic role of APC and RASSF1A promoter methylation status in cell free circulating DNA of operable gastric cancer patients.APC和RASSF1A启动子甲基化状态在可手术胃癌患者游离循环DNA中的预后作用
Mutat Res. 2015 Aug;778:46-51. doi: 10.1016/j.mrfmmm.2015.05.002. Epub 2015 Jun 11.
4
Assessment of SOX17 DNA methylation in cell free DNA from patients with operable gastric cancer. Association with prognostic variables and survival.评估可手术胃癌患者游离 DNA 中的 SOX17 甲基化。与预后变量和生存的关系。
Clin Chem Lab Med. 2013 Jul;51(7):1505-10. doi: 10.1515/cclm-2012-0320.
5
Correlations of Promoter Methylation in WIF-1, RASSF1A, and CDH13 Genes with the Risk and Prognosis of Esophageal Cancer.WIF-1、RASSF1A和CDH13基因启动子甲基化与食管癌风险及预后的相关性
Med Sci Monit. 2016 Aug 10;22:2816-24. doi: 10.12659/msm.896877.
6
Predictive value of CHFR and MLH1 methylation in human gastric cancer.CHFR和MLH1甲基化在人胃癌中的预测价值。
Gastric Cancer. 2015 Apr;18(2):280-7. doi: 10.1007/s10120-014-0370-2. Epub 2014 Apr 21.
7
Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients.胃癌和结直肠癌患者血清中RASSF1A启动子高甲基化的检测
World J Gastroenterol. 2008 May 21;14(19):3074-80. doi: 10.3748/wjg.14.3074.
8
SOX17 promoter methylation in plasma circulating tumor DNA of patients with non-small cell lung cancer.非小细胞肺癌患者血浆循环肿瘤 DNA 中 SOX17 启动子甲基化。
Clin Chem Lab Med. 2016 Aug 1;54(8):1385-93. doi: 10.1515/cclm-2015-0776.
9
Promoter methylation of MGMT, MLH1 and RASSF1A tumor suppressor genes in head and neck squamous cell carcinoma: pharmacological genome demethylation reduces proliferation of head and neck squamous carcinoma cells.MGMT、MLH1 和 RASSF1A 肿瘤抑制基因启动子甲基化与头颈部鳞状细胞癌：药物基因组去甲基化可降低头颈部鳞状细胞癌细胞的增殖。
Oncol Rep. 2012 Apr;27(4):1135-41. doi: 10.3892/or.2012.1624. Epub 2012 Jan 9.
10
Association of diminished expression of RASSF1A with promoter methylation in primary gastric cancer from patients of central China.中国中部地区原发性胃癌中RASSF1A表达降低与启动子甲基化的关联
BMC Cancer. 2007 Jul 3;7:120. doi: 10.1186/1471-2407-7-120.

引用本文的文献

1
Role of circulating tumor DNA methylation in gastric cancer initiation and progression: A comprehensive review.循环肿瘤DNA甲基化在胃癌发生和发展中的作用：综述
World J Gastrointest Oncol. 2025 Aug 15;17(8):107412. doi: 10.4251/wjgo.v17.i8.107412.
2
Diagnostic performance of SHOX2 and RASSF1A gene methylation assays in malignant pleural effusion: A systematic review and meta-analysis.SHOX2和RASSF1A基因甲基化检测在恶性胸腔积液中的诊断性能：一项系统评价和荟萃分析
Cancer Cytopathol. 2025 Aug;133(8):e70031. doi: 10.1002/cncy.70031.
3
Regulation of immune-mediated chemoresistance in cancer by lncRNAs: an in-depth review of signaling pathways.

本文引用的文献

1
Cancer statistics, 2020.癌症统计数据，2020 年。
CA Cancer J Clin. 2020 Jan;70(1):7-30. doi: 10.3322/caac.21590. Epub 2020 Jan 8.
2
Characterization of the release and biological significance of cell-free DNA from breast cancer cell lines.乳腺癌细胞系游离DNA的释放特征及其生物学意义
Oncotarget. 2017 Jun 27;8(26):43180-43191. doi: 10.18632/oncotarget.17858.
3
DNA Methylation in Cancer and Aging.癌症与衰老中的 DNA 甲基化。
长链非编码RNA对癌症中免疫介导的化疗耐药性的调控：信号通路深入综述
Naunyn Schmiedebergs Arch Pharmacol. 2025 Apr 9. doi: 10.1007/s00210-025-04081-3.
4
Extensive methylation analysis of circulating tumor DNA in plasma of patients with gastric cancer.胃癌患者血浆中循环肿瘤DNA的广泛甲基化分析
Sci Rep. 2024 Dec 28;14(1):30739. doi: 10.1038/s41598-024-79252-y.
5
Liquid biopsy in cancer current: status, challenges and future prospects.癌症液体活检的现状、挑战与未来前景
Signal Transduct Target Ther. 2024 Dec 2;9(1):336. doi: 10.1038/s41392-024-02021-w.
6
Clinical Applications of Circulating Tumor DNA Profiling in GI Cancers.循环肿瘤 DNA 分析在胃肠道癌症中的临床应用。
JCO Oncol Pract. 2024 Nov;20(11):1481-1490. doi: 10.1200/OP.24.00167. Epub 2024 Nov 12.
7
Circulating tumor DNA methylation detection as biomarker and its application in tumor liquid biopsy: advances and challenges.循环肿瘤DNA甲基化检测作为生物标志物及其在肿瘤液体活检中的应用：进展与挑战
MedComm (2020). 2024 Nov 9;5(11):e766. doi: 10.1002/mco2.766. eCollection 2024 Nov.
8
Circulating tumor cells: from new biological insights to clinical practice.循环肿瘤细胞：从新的生物学见解到临床实践。
Signal Transduct Target Ther. 2024 Sep 2;9(1):226. doi: 10.1038/s41392-024-01938-6.
9
Kinetic Profiling of Mutations With Circulating Tumor DNA in the Canadian Cancer Trials Group CO.26 Trial Suggests the Loss of Mutations in Neo--Wildtype Metastatic Colorectal Cancer Is Transient.在加拿大癌症临床试验组 CO.26 试验中，利用循环肿瘤 DNA 进行的突变动力学分析表明，新野生型转移性结直肠癌中突变的丢失是暂时的。
JCO Precis Oncol. 2024 Aug;8:e2400031. doi: 10.1200/PO.24.00031.
10
Effects of DNA methylation and its application in inflammatory bowel disease (Review).DNA 甲基化的影响及其在炎症性肠病中的应用（综述）。
Int J Mol Med. 2024 Jun;53(6). doi: 10.3892/ijmm.2024.5379. Epub 2024 May 2.
Cancer Res. 2016 Jun 15;76(12):3446-50. doi: 10.1158/0008-5472.CAN-15-3278. Epub 2016 Jun 2.
4
The detective, prognostic, and predictive value of DNA methylation in human esophageal squamous cell carcinoma.DNA甲基化在人食管鳞状细胞癌中的诊断、预后及预测价值。
Clin Epigenetics. 2016 Apr 22;8:43. doi: 10.1186/s13148-016-0210-9. eCollection 2016.
5
Epigenetic Biomarkers for the Early Detection of Gastrointestinal Cancer.用于胃肠道癌早期检测的表观遗传生物标志物
Gastrointest Tumors. 2014 May;1(4):201-8. doi: 10.1159/000380784. Epub 2015 Apr 15.
6
Prognostic role of APC and RASSF1A promoter methylation status in cell free circulating DNA of operable gastric cancer patients.APC和RASSF1A启动子甲基化状态在可手术胃癌患者游离循环DNA中的预后作用
Mutat Res. 2015 Aug;778:46-51. doi: 10.1016/j.mrfmmm.2015.05.002. Epub 2015 Jun 11.
7
Dietary patterns and gastric cancer risk: a systematic review and meta-analysis.饮食模式与胃癌风险：系统评价和荟萃分析。
Ann Oncol. 2013 Jun;24(6):1450-8. doi: 10.1093/annonc/mdt108. Epub 2013 Mar 22.
8
Assessment of SOX17 DNA methylation in cell free DNA from patients with operable gastric cancer. Association with prognostic variables and survival.评估可手术胃癌患者游离 DNA 中的 SOX17 甲基化。与预后变量和生存的关系。
Clin Chem Lab Med. 2013 Jul;51(7):1505-10. doi: 10.1515/cclm-2012-0320.
9
CpG island methylator phenotype and Helicobacter pylori infection associated with gastric cancer.CpG 岛甲基化表型与幽门螺杆菌感染与胃癌相关。
World J Gastroenterol. 2012 Sep 28;18(36):5129-34. doi: 10.3748/wjg.v18.i36.5129.
10
Quantitative assessment of gene methylation and their impact on clinical outcome in gastric cancer.胃癌中基因甲基化的定量评估及其对临床结局的影响。
Clin Chim Acta. 2012 Apr 11;413(7-8):787-94. doi: 10.1016/j.cca.2012.01.013. Epub 2012 Jan 21.