• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

中国中部地区原发性胃癌中RASSF1A表达降低与启动子甲基化的关联

Association of diminished expression of RASSF1A with promoter methylation in primary gastric cancer from patients of central China.

作者信息

Ye Mei, Xia Bing, Guo Qiusha, Zhou Feng, Zhang Xiaolian

机构信息

Department of Internal Medicine & Geriatrics, Research Centre of Digestive Diseases, Zhongnan Hospital, Wuhan University, PR China.

出版信息

BMC Cancer. 2007 Jul 3;7:120. doi: 10.1186/1471-2407-7-120.

DOI:10.1186/1471-2407-7-120
PMID:17608924
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1925110/
Abstract

BACKGROUND

Although methylation-mediated inactivation of expression of RASSF1A, a candidate tumor suppressor gene, has been observed in several human cancers, the data concerning alteration of RASSF1A expression and methylation in Chinese primary gastric cancer are scarce. Moreover, direct evidence showing the association between protein expression of RASSF1A and primary human cancers is lacking. The aim of this study was to investigate RASSF1A expression in tissue of primary gastric cancer (GC) at mRNA and protein levels, and to establish the possible relationship between DNA methylation status and protein expression of RASSF1A in Chinese.

METHODS

Fifty-four patients with primary gastric cancers were included in the study of RASSF1A mRNA expression and methylation status between the cancer tissue and the corresponding adjacent normal tissue. 20 out of 54 patients were included for study of RASSF1A protein expression. The expression of RASSF1A at mRNA and protein levels was determined by RT-PCR and Western-blotting, respectively. The RASSF1A promoter methylation was detected by methylation-specific PCR.

RESULTS

RASSF1A mRNA and protein expressions in GC were reduced significantly with comparison to the corresponding normal tissues (OD value: 0.2589 +/- 0.2407 vs 0.5448 +/- 0.2971, P < 0.0001; 0.1874 +/- 0.0737 vs 0.6654 +/- 0.2201, P < 0.0001, respectively). Methylation frequency of RASSF1A in primary GC is higher than that in the corresponding normal tissues (66.7% vs. 14.8%, P < 0.0001). Furthermore, RASSF1A mRNA expression in methylation group of GC was further reduced when compared to the unmethylation group of GC (0.1384 +/- 0.1142 vs. 0.5018 +/- 0.2463, P < 0.0001).

CONCLUSION

Expression of RASSF1A was reduced in tissue of GC at mRNA and protein levels. Diminished expression of RASSF1A was associated with the promoter methylation.

摘要

背景

尽管在几种人类癌症中已观察到候选抑癌基因RASSF1A的甲基化介导的表达失活，但关于中国原发性胃癌中RASSF1A表达和甲基化改变的数据却很少。此外，缺乏显示RASSF1A蛋白表达与原发性人类癌症之间关联的直接证据。本研究的目的是在mRNA和蛋白质水平上研究原发性胃癌（GC）组织中RASSF1A的表达，并在中国人群中确定RASSF1A的DNA甲基化状态与蛋白质表达之间的可能关系。

方法

54例原发性胃癌患者纳入研究，分析癌组织和相应癌旁正常组织中RASSF1A mRNA表达和甲基化状态。54例患者中的20例纳入RASSF1A蛋白表达研究。分别通过RT-PCR和Western印迹法测定RASSF1A在mRNA和蛋白质水平的表达。通过甲基化特异性PCR检测RASSF1A启动子甲基化。

结果

与相应正常组织相比，GC中RASSF1A mRNA和蛋白质表达显著降低（OD值：0.2589±0.2407对0.5448±0.2971，P<0.0001；0.1874±0.0737对0.6654±0.2201，P<0.0001）。原发性GC中RASSF1A的甲基化频率高于相应正常组织（66.7%对14.8%，P<0.0001）。此外，与GC未甲基化组相比，GC甲基化组中RASSF1A mRNA表达进一步降低（0.1384±0.1142对0.5018±0.2463，P<0.0001）。

结论

GC组织中RASSF1A在mRNA和蛋白质水平表达降低。RASSF1A表达降低与启动子甲基化有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/6769c055d06c/1471-2407-7-120-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/86b040b853de/1471-2407-7-120-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/772243f16e9f/1471-2407-7-120-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/6769c055d06c/1471-2407-7-120-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/86b040b853de/1471-2407-7-120-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/772243f16e9f/1471-2407-7-120-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b2bc/1925110/6769c055d06c/1471-2407-7-120-3.jpg

相似文献

1
Association of diminished expression of RASSF1A with promoter methylation in primary gastric cancer from patients of central China.中国中部地区原发性胃癌中RASSF1A表达降低与启动子甲基化的关联
BMC Cancer. 2007 Jul 3;7:120. doi: 10.1186/1471-2407-7-120.
2
Aberrant CpG island hypermethylation of RASSF1A in gastric cardia adenocarcinoma.贲门腺癌中RASSF1A基因异常的CpG岛高甲基化
Cancer Invest. 2009 May;27(4):459-65. doi: 10.1080/07357900802620828.
3
[The influence of promoter methylation of RASSF1A on RASSF1A mRNA and protein expression in gastric cancer tissues].[RASSF1A基因启动子甲基化对胃癌组织中RASSF1A mRNA及蛋白表达的影响]
Zhonghua Nei Ke Za Zhi. 2006 Dec;45(12):1008-12.
4
Polymorphism of A133S and promoter hypermethylation in Ras association domain family 1A gene (RASSF1A) is associated with risk of esophageal and gastric cardia cancers in Chinese population from high incidence area in northern China.A133S 多态性和 Ras 相关结构域家族 1A 基因（RASSF1A）启动子超甲基化与中国北方高发地区人群食管和贲门癌的风险相关。
BMC Cancer. 2013 May 25;13:259. doi: 10.1186/1471-2407-13-259.
5
Prognostic impact of expression and methylation status of DENN/MADD domain-containing protein 2D in gastric cancer.胃癌中 DENN/MADD 结构域蛋白 2D 的表达和甲基化状态对预后的影响。
Gastric Cancer. 2015 Apr;18(2):288-96. doi: 10.1007/s10120-014-0372-0. Epub 2014 Apr 3.
6
Detection of RASSF1A promoter hypermethylation in serum from gastric and colorectal adenocarcinoma patients.胃癌和结直肠癌患者血清中RASSF1A启动子高甲基化的检测
World J Gastroenterol. 2008 May 21;14(19):3074-80. doi: 10.3748/wjg.14.3074.
7
Epigenetic regulation of death-associated protein kinase expression in primary gastric cancers from Chinese patients.中国原发性胃癌中死亡相关蛋白激酶表达的表观遗传调控。
Eur J Cancer Prev. 2012 May;21(3):241-6. doi: 10.1097/CEJ.0b013e32834c9caa.
8
Promoter hypermethylation of multiple genes in early gastric adenocarcinoma and precancerous lesions.早期胃腺癌及癌前病变中多个基因的启动子高甲基化
Hum Pathol. 2009 Nov;40(11):1534-42. doi: 10.1016/j.humpath.2009.01.029. Epub 2009 Aug 19.
9
Prognostic role of APC and RASSF1A promoter methylation status in cell free circulating DNA of operable gastric cancer patients.APC和RASSF1A启动子甲基化状态在可手术胃癌患者游离循环DNA中的预后作用
Mutat Res. 2015 Aug;778:46-51. doi: 10.1016/j.mrfmmm.2015.05.002. Epub 2015 Jun 11.
10
Prognostic Role of RASSF1A, SOX17 and Wif-1 Promoter Methylation Status in Cell-Free DNA of Advanced Gastric Cancer Patients.晚期胃癌患者循环游离 DNA 中 RASSF1A、SOX17 和 Wif-1 启动子甲基化状态的预后作用。
Technol Cancer Res Treat. 2021 Jan-Dec;20:1533033820973279. doi: 10.1177/1533033820973279.

引用本文的文献

1
Methylation Analysis of and in Circulating Cell-Free DNA for Detection of Gastric Cancer: A Validation Study.循环游离DNA中用于胃癌检测的[具体基因]甲基化分析：一项验证研究 （你提供的原文中存在信息缺失，这里的“and”后面应该有具体基因名称）
Avicenna J Med Biotechnol. 2020 Apr-Jun;12(2):99-106.
2
Combined Detection of Plasma ZIC1, HOXD10 and RUNX3 Methylation is a Promising Strategy for Early Detection of Gastric Cancer and Precancerous Lesions.联合检测血浆ZIC1、HOXD10和RUNX3甲基化是早期检测胃癌及癌前病变的一种有前景的策略。
J Cancer. 2017 Apr 8;8(6):1038-1044. doi: 10.7150/jca.18169. eCollection 2017.
3
Diminished expression of & genes in gastric cancer in ethnic population of Kashmir.

本文引用的文献

1
Tumor susceptibility of Rassf1a knockout mice.Rassf1a基因敲除小鼠的肿瘤易感性。
Cancer Res. 2005 Jan 1;65(1):92-8.
2
Tumor suppressor RASSF1A is a microtubule-binding protein that stabilizes microtubules and induces G2/M arrest.肿瘤抑制因子RASSF1A是一种微管结合蛋白，可使微管稳定并诱导G2/M期阻滞。
Oncogene. 2004 Oct 28;23(50):8216-30. doi: 10.1038/sj.onc.1207901.
3
A role for the RASSF1A tumor suppressor in the regulation of tubulin polymerization and genomic stability.RASSF1A肿瘤抑制因子在微管蛋白聚合和基因组稳定性调控中的作用。
克什米尔族人群胃癌中&基因表达降低。 （注：原文中“&”指代不明，这里按原样翻译）
J Gastrointest Oncol. 2016 Dec;7(6):989-995. doi: 10.21037/jgo.2016.06.07.
4
Aberrant methylation of PCDH10 and RASSF1A genes in blood samples for non-invasive diagnosis and prognostic assessment of gastric cancer.血液样本中PCDH10和RASSF1A基因的异常甲基化用于胃癌的非侵入性诊断和预后评估
PeerJ. 2016 Jun 9;4:e2112. doi: 10.7717/peerj.2112. eCollection 2016.
5
Detection of OSR2, VAV3, and PPFIA3 Methylation in the Serum of Patients with Gastric Cancer.胃癌患者血清中OSR2、VAV3和PPFIA3甲基化的检测
Dis Markers. 2016;2016:5780538. doi: 10.1155/2016/5780538. Epub 2016 Apr 7.
6
Methylation status of TRAF2 is associated with the diagnosis and prognosis of gastric cancer.TRAF2的甲基化状态与胃癌的诊断和预后相关。
Int J Clin Exp Pathol. 2015 Nov 1;8(11):14228-34. eCollection 2015.
7
RASSF1A inhibits gastric cancer cell proliferation by miR-711- mediated downregulation of CDK4 expression.RASSF1A通过miR-711介导的CDK4表达下调来抑制胃癌细胞增殖。
Oncotarget. 2016 Feb 2;7(5):5842-51. doi: 10.18632/oncotarget.6813.
8
Clinical Significance of MLH1 Methylation and CpG Island Methylator Phenotype as Prognostic Markers in Patients with Gastric Cancer.MLH1甲基化和CpG岛甲基化表型作为胃癌患者预后标志物的临床意义
PLoS One. 2015 Jun 29;10(6):e0130409. doi: 10.1371/journal.pone.0130409. eCollection 2015.
9
Association of RASSF1A promoter methylation with gastric cancer risk: a meta-analysis.RASSF1A基因启动子甲基化与胃癌风险的关联：一项荟萃分析。
Tumour Biol. 2014 Feb;35(2):943-8. doi: 10.1007/s13277-013-1123-2. Epub 2013 Aug 28.
10
Genomic and epigenetic profiles of gastric cancer: potential diagnostic and therapeutic applications.胃癌的基因组和表观基因组特征：潜在的诊断和治疗应用。
Surg Today. 2011 Jan;41(1):24-38. doi: 10.1007/s00595-010-4370-5. Epub 2010 Dec 30.
Cancer Res. 2004 Jun 15;64(12):4244-50. doi: 10.1158/0008-5472.CAN-04-0339.
4
RASSF1A interacts with microtubule-associated proteins and modulates microtubule dynamics.RASSF1A与微管相关蛋白相互作用并调节微管动力学。
Cancer Res. 2004 Jun 15;64(12):4112-6. doi: 10.1158/0008-5472.CAN-04-0267.
5
Haploinsufficiency for tumour suppressor genes: when you don't need to go all the way.肿瘤抑制基因的单倍剂量不足：何时无需完全失活。
Biochim Biophys Acta. 2004 Jun 7;1654(2):105-22. doi: 10.1016/j.bbcan.2004.01.001.
6
Reduced expression of death-associated protein kinase in human uterine and ovarian carcinoma cells.死亡相关蛋白激酶在人子宫和卵巢癌细胞中的表达降低。
Oncol Rep. 2004 Mar;11(3):661-5.
7
The tumour suppressor RASSF1A regulates mitosis by inhibiting the APC-Cdc20 complex.肿瘤抑制因子RASSF1A通过抑制后期促进复合体/细胞周期蛋白依赖性激酶20（APC-Cdc20）复合体来调控有丝分裂。
Nat Cell Biol. 2004 Feb;6(2):129-37. doi: 10.1038/ncb1091. Epub 2004 Jan 25.
8
Control of microtubule stability by the RASSF1A tumor suppressor.肿瘤抑制因子RASSF1A对微管稳定性的调控
Oncogene. 2003 Nov 6;22(50):8125-36. doi: 10.1038/sj.onc.1206984.
9
Aberrant CpG island hypermethylation of chronic gastritis, in relation to aging, gender, intestinal metaplasia, and chronic inflammation.慢性胃炎异常的CpG岛高甲基化与衰老、性别、肠化生及慢性炎症的关系
Am J Pathol. 2003 Oct;163(4):1551-6. doi: 10.1016/S0002-9440(10)63511-0.
10
Profile of aberrant CpG island methylation along multistep gastric carcinogenesis.多步骤胃癌发生过程中异常CpG岛甲基化图谱。
Lab Invest. 2003 Apr;83(4):519-26. doi: 10.1097/01.lab.0000064704.53132.65.