Radiation Biology Department, National Centre for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy Authority, Cairo, Egypt.
J Radiat Res. 2021 Jul 10;62(4):600-617. doi: 10.1093/jrr/rraa141.
Apelin-13 and APJ are implicated in different key physiological processes. This work aims at exploring the radioprotective effect of fucoxanthin (FX) on γ-radiation (RAD)-induced changes in the apelin-13/APJ pathway, which causes damage in the liver, kidney, lung and spleen of mice. Mice were administered FX (10 mg kg-1 day-1, i.p) and exposed to γ-radiation (2.5 Gy week-1) for four consecutive weeks. The treatment of irradiated mice by FX resulted in a significant amendment in protein expression of the apelin-13/APJ/NF-κB signalling pathway concurrently with reduced hypoxia (hypoxia-inducible factor-1α), suppressed oxidative stress marker (malondialdehyde), enhanced antioxidant defence mechanisms (reduced glutathione and glutathione peroxidase), a modulated inflammatory response [interleukin-6 (IL-6), monocyte chemoattractant protein-1, IL-10 and α-7-nicotinic acetylcholine receptor) and ameliorated angiogenic regulators [matrix metalloproteinase (MMP-2), MMP-9 and tissue inhibitor of metalloproteinase-1), as well as the tissue damage indicator (lactate dehydrogenase) in organ tissues. In addition, there were significant improvement in serum inflammatory markers tumour necrosis factor-α, IL-10, IL-1β and C-reactive protein compared with irradiated mice. The histopathological investigation of the FX + RAD organ tissues support the biochemical findings where the improvements in the tissues' architecture were obvious when compared with those of RAD. FX was thus shown to have a noticeable radioprotective action mediated through its regulatory effect on the apelin-13/APJ/NF-κB signalling pathway attributed to its antioxidant and anti-inflammatory activity that was reflected in different physiological processes. It could be recommended to use FX in cases of radiation exposure to protect normal tissues.
Apelin-13 和 APJ 参与不同的关键生理过程。本研究旨在探讨岩藻黄质 (FX) 对 γ 射线 (RAD) 诱导的肝、肾、肺和脾损伤小鼠中 apelin-13/APJ 通路的放射保护作用。小鼠给予 FX（10 mg kg-1 day-1，腹腔注射），并连续 4 周每周接受 γ 射线（2.5 Gy）照射。FX 处理照射小鼠可显著改善 apelin-13/APJ/NF-κB 信号通路的蛋白表达，同时降低缺氧（缺氧诱导因子-1α）、抑制氧化应激标志物（丙二醛）、增强抗氧化防御机制（还原型谷胱甘肽和谷胱甘肽过氧化物酶）、调节炎症反应 [白细胞介素-6 (IL-6)、单核细胞趋化蛋白-1、白细胞介素-10 和 α-7-烟碱型乙酰胆碱受体）] 和改善血管生成调节剂 [基质金属蛋白酶 (MMP-2)、MMP-9 和金属蛋白酶组织抑制剂-1)，以及组织损伤标志物（乳酸脱氢酶）在器官组织中的表达。此外，与照射小鼠相比，血清炎症标志物肿瘤坏死因子-α、IL-10、IL-1β 和 C 反应蛋白的水平也显著改善。FX 处理 + RAD 组织的组织病理学检查支持生化发现，与 RAD 相比，组织结构的改善更加明显。因此，FX 具有显著的放射保护作用，这与其抗氧化和抗炎活性有关，通过调节 apelin-13/APJ/NF-κB 信号通路来保护正常组织。在遭受辐射的情况下，可以建议使用 FX 来保护正常组织。
