Allergy Department, State Key Laboratory of Complex Severe and Rare Diseases, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100730, China.
Food Funct. 2021 May 21;12(10):4707-4719. doi: 10.1039/d1fo00335f. Epub 2021 Apr 30.
We previously described that Porphyra haitanensis sulfated polysaccharide (PHSP) maintains the balance of pro-inflammation and immunosuppression. However, it is unclear whether degraded PHSP (DPHSP) still shows the immunomodulatory activity. Here, we degraded PHSP by four different methods alone or combined in pairs, and the results showed that the molecular weight and viscosity of DPHSP were significantly decreased, while the main chemical bonds and functional structure were consistent with those of PHSP. We then investigated the immunomodulatory function of DPHSP in vitro and in vivo. Actually, DPHSP enhances the inhibitory effects on mast cell activation and improves the suppression activity of PHSP on the food anaphylactic response. In an ovalbumin-induced food allergy mouse model, the production of allergic mediators and cytokines (interleukin-4 and 13, and interferon-γ) was inhibited by DPHSP. Meanwhile, DPHSP had a stronger ability to up-regulate the differentiation of regulatory T (Treg) cells and its related cytokines. These results suggested that DPHSP showed a better anti-food allergic ability than PHSP by regulating T helper cell balance and promoting Treg cell differentiation, which indicates that DPHSP is a novel potential nutrient component against food allergy.
我们之前描述过海白菜硫酸多糖（PHSP）可以维持促炎与免疫抑制之间的平衡。然而，其降解产物（DPHSP）是否仍然具有免疫调节活性尚不清楚。在此，我们单独或两两组合使用四种不同方法对 PHSP 进行降解，结果表明 DPHSP 的分子量和黏度显著降低，而主要化学键和功能结构与 PHSP 一致。随后，我们在体外和体内研究了 DPHSP 的免疫调节功能。实际上，DPHSP 增强了对肥大细胞活化的抑制作用，并提高了 PHSP 对食物过敏反应的抑制活性。在卵清蛋白诱导的食物过敏小鼠模型中，DPHSP 抑制了过敏介质和细胞因子（白细胞介素-4 和白细胞介素-13、干扰素-γ）的产生。同时，DPHSP 具有更强的能力来上调调节性 T（Treg）细胞及其相关细胞因子的分化。这些结果表明，DPHSP 通过调节辅助性 T 细胞平衡和促进 Treg 细胞分化，表现出比 PHSP 更好的抗食物过敏能力，这表明 DPHSP 是一种新型的潜在抗食物过敏营养成分。
