坛紫菜硫酸多糖的体内外免疫调节活性。
In vitro and in vivo immunomodulatory activity of sulfated polysaccharide from Porphyra haitanensis.
机构信息
College of Food and Biological Engineering, Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources, Jimei University, 43 Yindou Road, Xiamen 361021, Fujian, PR China.
Department of Biochemical Science and Technology, College of Life Science, National Taiwan University, No. 1, Sec. 4, Roosevelt Road, Taipei 10617, Taiwan.
出版信息
Carbohydr Polym. 2017 Jun 1;165:189-196. doi: 10.1016/j.carbpol.2017.02.032. Epub 2017 Feb 14.
The immunoregulatory activity of sulfated polysaccharide from Porphyra haitanensis (PHPS) was investigated in a RAW264.7 macrophages cell model and a BALB/c murine model. The subpopulation of dendritic cells (DCs) and regulatory T cells (Tregs) from PHPS-treated mice splenocytes were also measured by flow cytometry. Consistent with previous reports, we showed that PHPS increased the phagocytosis of RAW264.7 macrophages, and enhanced the secretion of interleukin (IL)-6, IL-10 and tumor necrosis factor-α (TNF-α). Meanwhile, PHPS induced the production of nitric oxide via the Jun N-terminal kinase (JNK) and the Janus kinase (JAK2) signaling pathways in RAW264.7 macrophages. Furthermore, PHPS promoted the proliferation of mice lymphocytes, inducing the generation of TNF-α and IL-10 in vivo, as well as the subpopulation of CD4+ splenic T lymphocytes, DCs, and Tregs. These results indicated that PHPS plays key roles in immunoregulation and may be apply to develop new health foods.
本研究以坛紫菜（Porphyra haitanensis）硫酸多糖（PHPS）为研究对象，探讨其在 RAW264.7 巨噬细胞细胞模型和 BALB/c 小鼠模型中的免疫调节活性。通过流式细胞术检测 PHPS 处理的小鼠脾细胞中树突状细胞（DC）和调节性 T 细胞（Treg）亚群。与先前的报道一致，我们发现 PHPS 能增强 RAW264.7 巨噬细胞的吞噬作用，并促进白细胞介素（IL）-6、IL-10 和肿瘤坏死因子-α（TNF-α）的分泌。同时，PHPS 通过丝裂原活化蛋白激酶（MAPK）中的 c-Jun N 端激酶（JNK）和 Janus 激酶（JAK2）信号通路诱导 RAW264.7 巨噬细胞产生一氧化氮。此外，PHPS 促进小鼠淋巴细胞的增殖，在体内诱导 TNF-α和 IL-10 的产生，以及 CD4+脾 T 淋巴细胞、DC 和 Treg 的亚群。这些结果表明 PHPS 在免疫调节中发挥重要作用，可能适用于开发新的保健品。
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