Department of Diagnostics and Microsurgery of Glaucoma, Medical University of Lublin, ul. Chmielna 1, 20-079, Lublin, Poland.
Department of Clinical Genetics, Medical University of Lublin, ul. Radziwiłłowska 11, 20-080, Lublin, Poland.
Graefes Arch Clin Exp Ophthalmol. 2021 Aug;259(8):2337-2349. doi: 10.1007/s00417-021-05214-z. Epub 2021 Apr 30.
The aim of the study was to compare the frequency and the level of expression of selected miRNAs in the aqueous humor of patients with various types of glaucoma.
The studied group consisted of 42 patients with glaucoma: 19 with primary open-angle glaucoma (POAG), 14 with pseudoexfoliation glaucoma (PEXG), 9 with primary angle closure glaucoma (PACG), and the control group of 36 patients with senile cataract without glaucoma. The real-time polymerase chain reaction method was used to analyze the expression of miRNAs.
There were no significant differences in the frequency and the level of miRNA expression between various types of glaucoma. There was a tendency for hsa-miR-6722-3p and hsa-miR-184 to be expressed more frequently in PEXG and hsa-miR-1260b in POAG. The expression levels of hsa-miR-1260b and hsa-miR-6515-3p were correlated with age in POAG. Target annotation and functional analyses showed that genes targeted by the most frequently expressed miRNAs (hsa-miR-1202, -1260b, -184, -187-5p, -6515-3p, -6722-3p, and hsa-mir-4634) are involved mainly in response to hypoxia, cardiovascular system development, and apoptosis.
Hsa-miR-1260b was the most abundantly expressed among studied miRNAs and may be a potential biomarker of clinical status in PEXG and PACG.
本研究旨在比较不同类型青光眼患者房水中选定 miRNA 的表达频率和水平。
研究组包括 42 名青光眼患者：19 名原发性开角型青光眼（POAG）患者，14 名假性剥脱性青光眼（PEXG）患者，9 名原发性闭角型青光眼（PACG）患者，对照组为 36 名无青光眼的老年性白内障患者。采用实时聚合酶链反应方法分析 miRNA 的表达。
各种类型青光眼之间 miRNA 的表达频率和水平无显著差异。hsa-miR-6722-3p 和 hsa-miR-184 在 PEXG 中表达更为频繁，hsa-miR-1260b 在 POAG 中表达更为频繁。POAG 中 hsa-miR-1260b 和 hsa-miR-6515-3p 的表达水平与年龄相关。靶基因注释和功能分析表明，表达频率最高的 miRNA（hsa-miR-1202、-1260b、-184、-187-5p、-6515-3p、-6722-3p 和 hsa-mir-4634）的靶基因主要参与缺氧反应、心血管系统发育和细胞凋亡。
hsa-miR-1260b 在研究的 miRNA 中表达最丰富，可能是 PEXG 和 PACG 临床状态的潜在生物标志物。
