College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China.
College of Food and Biological Engineering, Jimei University, Xiamen, 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Xiamen, 361021, China.
Biosens Bioelectron. 2021 Aug 1;185:113278. doi: 10.1016/j.bios.2021.113278. Epub 2021 Apr 24.
The long-term toxic effects of okadaic acid (OA) in shellfish pose a serious threat to public health, negatively impacting the development of the shellfish aquaculture industry. In this study, a novel competitive near-infrared-mediated photoelectrochemical immunosorbent assay (cNIR-PECIA) was developed for ultrasensitive and highly selective detection of OA based on NaYF:Yb, Tm upconversion nanophosphors (UCNPs) and a flower-like WO-modified screen-printed electrode (FL-WO SPE). The UCNPs function as a self-powder to convert NIR excitation into visible emissions. FL-WO fully utilizes the visible illumination and induces the separation of electron-hole pairs, thus generating a photocurrent. After conjugating monoclonal antibodies against OA on UCNPs (UCNPs-Ab), the bright PEC immunoprobe selectively captured OA molecules, which were then determined by a competitive indirect immunosorbent assay. Under optimal conditions, the 50% inhibitory concentration of the immunosensor was 0.09 ng mL. The OA concentration had a linear relationship with the antibody binding rate in the range of 0.01-60 ng mL with an extremely low detection limit of 0.007 ng mL. Finally, the proposed cNIR-PECIA was successfully utilized to analyze OA content in mussel samples. This study affords new ideas for constructing NIR PEC sensors by using upconversion luminescent materials to match semiconductors. The superior sensing properties indicate their potential applicability in food safety analysis.
贝类中 okadaic 酸（OA）的长期毒性效应对公众健康构成严重威胁，对贝类养殖业的发展产生负面影响。在本研究中，基于上转换纳米荧光粉（UCNPs）和花状 WO 修饰的丝网印刷电极（FL-WO SPE），开发了一种新颖的竞争性近红外介导光电流免疫分析（cNIR-PECIA），用于超灵敏和高选择性检测 OA。UCNPs 用作将 NIR 激发转换为可见发射的自粉。FL-WO 充分利用可见光照射并诱导电子-空穴对的分离，从而产生光电流。在 UCNPs（UCNPs-Ab）上缀合针对 OA 的单克隆抗体后，明亮的 PEC 免疫探针选择性地捕获 OA 分子，然后通过竞争性间接免疫吸附测定进行测定。在最佳条件下，免疫传感器的 50%抑制浓度为 0.09ng mL。OA 浓度与抗体结合率在 0.01-60ng mL 范围内呈线性关系，检测限极低，为 0.007ng mL。最后，成功地将所提出的 cNIR-PECIA 用于贻贝样品中 OA 含量的分析。本研究为利用上转换发光材料与半导体匹配构建近红外 PEC 传感器提供了新的思路。优越的传感性能表明它们在食品安全分析中具有潜在的适用性。
