用于同时测定海兔科中河豚毒素和 okadaic 酸的 NIR 响应光电流化学传感平台。
NIR-responsive photoelectrochemical sensing platform for the simultaneous determination of tetrodotoxin and okadaic acid in Nassariidae.
机构信息
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China.
College of Ocean Food and Biological Engineering, Jimei University, Xiamen 361021, China; Fujian Provincial Key Laboratory of Food Microbiology and Enzyme Engineering, Jimei University, Xiamen 361021, China.
出版信息
Food Chem. 2024 Jan 1;430:136999. doi: 10.1016/j.foodchem.2023.136999. Epub 2023 Jul 29.
Simultaneous detection of tetrodotoxin (TTX) and okadaic acid (OA) is important for seafood safety. In this work, a novel paper electrode-based near-infrared (NIR) light-responsive photoelectrochemical (PEC) immunosensor was constructed using AgS quantum dots (QDs) and NaYF: Yb, Er upconversion nanoparticles (UCNPs) matched with BiOI for the simultaneous detection of TTX and OA in aquatic products. A low-cost, easily prepared gold nanoparticle-functionalized paper-based screen-printed electrode with six channels was designed to immobilize OA and Ab of TTX. Correspondingly, PEC signal immunoprobes (BiOI@UCNPs-Ab and Ab-AgS QDs) with NIR-light response were introduced to construct competitive-type and sandwich-type PEC immunosensors for OA and TTX, respectively. Under optimal conditions, the linear ranges for TTX and OA were 0.001-100 and 0.001-80 ng mL, respectively, and the detection limits were 5 and 7 pg mL, respectively. The proposed sensor was successfully used for the simultaneous analysis of TTX and OA in Nassariidae samples.
同时检测河豚毒素 (TTX) 和 okadaic 酸 (OA) 对海鲜安全很重要。在这项工作中，构建了一种基于新型纸电极的近红外 (NIR) 光响应光电化学 (PEC) 免疫传感器，该传感器使用 AgS 量子点 (QDs) 和与 BiOI 匹配的 NaYF:Yb，Er 上转换纳米粒子 (UCNPs) 用于同时检测水产品中的 TTX 和 OA。设计了一种低成本、易于制备的金纳米粒子功能化的具有六个通道的纸基丝网印刷电极来固定 OA 和 TTX 的 Ab。相应地，引入具有 NIR 光响应的 PEC 信号免疫探针 (BiOI@UCNPs-Ab 和 Ab-AgS QDs)，分别构建用于 OA 和 TTX 的竞争性和三明治型 PEC 免疫传感器。在最佳条件下，TTX 和 OA 的线性范围分别为 0.001-100 和 0.001-80 ng mL，检测限分别为 5 和 7 pg mL。该传感器成功用于 Nassariidae 样品中 TTX 和 OA 的同时分析。
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