Marubayashi S, Oku J, Dohi K, Ochi K, Yamada K, Kawasaki T
Department of Surgery, Hiroshima University School of Medicine, Japan.
Nihon Geka Gakkai Zasshi. 1988 Mar;89(3):381-7.
The present study was undertaken to determine whether CoQ10 pretreatment could modify cellular free radical metabolism in regenerating rat liver and enhance hepatic DNA synthesis. Male Wistar rats (250-300 g) were used. Partial 70% hepatectomy was performed according to Higgins and Anderson. CoQ10 (6 mg/kg body weight) and its solvent as a placebo were injected intravenously 1 hour before operation. Twenty-four hours or 48 hours after 70% hepatectomy resulted in 55% or 50% decreases of hepatic ATP, and marked decreases of endogenous CoQ homologs (CoQ9 and CoQ10), alpha-tocopherol and reduced glutathione (GSH) in remnant liver and was accompanied by 2.5-fold increases in lipid peroxides. However, in CoQ10-treated animals synthesis of ATP was accelerated after 48 hours after partial hepatectomy. There were no changes in the levels of CoQ9, alpha-tocopherol, or GSH or in the level of the enhanced CoQ10 in regenerating liver. The pretreatment also completely suppressed the elevation of lipid peroxide. Moreover, CoQ10 administration markedly increased hepatic DNA synthesis at 48 hours after partial hepatectomy. These results indicate that cellular damage during hepatic regeneration is in part due to lipid peroxidation, and suggest that CoQ10 is effective in the pretreatment of liver resection.
本研究旨在确定辅酶Q10预处理是否能改变再生大鼠肝脏中的细胞自由基代谢并增强肝脏DNA合成。使用雄性Wistar大鼠（250 - 300克）。根据希金斯和安德森的方法进行70%部分肝切除术。在手术前1小时静脉注射辅酶Q10（6毫克/千克体重）及其作为安慰剂的溶剂。70%肝切除术后24小时或48小时，残余肝脏中的肝ATP减少了55%或50%，内源性辅酶Q同系物（辅酶Q9和辅酶Q10）、α-生育酚和还原型谷胱甘肽（GSH）显著减少，并伴有脂质过氧化物增加2.5倍。然而，在接受辅酶Q10治疗的动物中，部分肝切除术后48小时ATP的合成加速。再生肝脏中辅酶Q9、α-生育酚或GSH的水平以及增强的辅酶Q10水平均无变化。预处理还完全抑制了脂质过氧化物的升高。此外，在部分肝切除术后48小时给予辅酶Q10显著增加了肝脏DNA合成。这些结果表明，肝脏再生过程中的细胞损伤部分归因于脂质过氧化，并提示辅酶Q10在肝切除术前预处理中是有效的。
