Barraj Barraj Rida, Segado Patricia, Moreno-González Rocío, Heredia Antonio, Fernández-Muñoz Rafael, Domínguez Eva
Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Mejora Genética y Biotecnología, Estación Experimental La Mayora, Algarrobo-Costa, E-29750, Málaga, Spain.
Instituto de Hortofruticultura Subtropical y Mediterránea La Mayora, Universidad de Málaga-Consejo Superior de Investigaciones Científicas, Departamento de Biología Molecular y Bioquímica, Universidad de Málaga, E-29071, Málaga, Spain.
Hortic Res. 2021 May 1;8(1):113. doi: 10.1038/s41438-021-00548-5.
Genetics of traits related to fruit cuticle deposition and composition was studied in two red-fruited tomato species. Two mapping populations derived from the cross between the cultivated tomato (Solanum lycopersicum L.) and its closest relative wild species Solanum pimpinellifolium L. were employed to conduct a QTL analysis. A combination of fruit cuticle deposition, components and anatomical traits were investigated and the individual effect of each QTL evaluated. A total of 70 QTLs were identified, indicating that all the cuticle traits analyzed have a complex polygenic nature. A combination of additive and epistatic interactions was observed for all the traits, with positive contribution of both parental lines to most of them. Colocalization of QTLs for various traits uncovered novel genomic regions producing extensive changes in the cuticle. Cuticle density emerges as an important trait since it can modulate cuticle thickness and invagination thus providing a strategy for sustaining mechanical strength without compromising palatability. Two genomic regions, located in chromosomes 1 and 12, are responsible for the negative interaction between cuticle waxes and phenolics identified in tomato fruit. Several candidate genes, including transcription factors and structural genes, are postulated and their expression analyzed throughout development.
在两个红果番茄品种中研究了与果实角质层沉积和组成相关性状的遗传学。利用两个由栽培番茄（Solanum lycopersicum L.）与其近缘野生种Solanum pimpinellifolium L.杂交产生的作图群体进行QTL分析。研究了果实角质层沉积、成分和解剖学性状的组合，并评估了每个QTL的个体效应。共鉴定出70个QTL，表明所分析的所有角质层性状都具有复杂的多基因性质。所有性状均观察到加性和上位性相互作用的组合，两个亲本系对大多数性状都有正向贡献。不同性状QTL的共定位揭示了在角质层中产生广泛变化的新基因组区域。角质层密度成为一个重要性状，因为它可以调节角质层厚度和内陷，从而提供一种在不影响适口性的情况下维持机械强度的策略。位于第1和第12号染色体上的两个基因组区域负责番茄果实中鉴定出的角质层蜡质和酚类物质之间的负向相互作用。推测了几个候选基因，包括转录因子和结构基因，并分析了它们在整个发育过程中的表达。
