Department of Infectious Diseases, Unit of Foodborne and Neglected Parasitic Diseases, Istituto Superiore di Sanità, Viale Regina Elena 299, 00161, Rome, Italy.
Istituto Zooprofilattico Sperimentale della Puglia e della Basilicata, Zona industriale Tito Scalo, 85050, Tito (PZ), Italy.
Parasitol Res. 2021 Jun;120(6):2287-2290. doi: 10.1007/s00436-021-07165-0. Epub 2021 May 1.
Trichinella pseudospiralis is a non-encapsulated species infecting both mammals and birds. In Italy, this species has been reported so far only in central regions (two nocturnal birds of prey, one red fox, and one wild boar) and in northeast regions (four wild boars). In November 2020, Trichinella sp. larvae were isolated by enzymatic digestion from muscle tissues of a red kite (Milvus milvus) specimen belonging to a population residing in the Basilicata region (Southern Italy). The parasite was identified as T. pseudospiralis by multiplex PCR, and the sequencing of the expansion segment V (ESV) region of the nuclear large subunit ribosomal DNA showed, in the microsatellite region, the polymorphism characteristic of the Palearctic population. This represents the first record of T. pseudospiralis in a red kite and the first report of this parasite in Southern Italy. The isolation of the parasite in a resident bird confirms that T. pseudospiralis is present, even if at low prevalence, in the Italian avifauna.
旋毛虫假型是一种既感染哺乳动物又感染鸟类的非包囊物种。在意大利，迄今为止，该物种仅在中部地区（两种夜间猛禽、一只赤狐和一只野猪）和东北部地区（四只野猪）有报道。2020 年 11 月，通过酶消化从栖息在意大利南部巴西利卡塔地区（Basilicata region）的红鸢（Milvus milvus）标本的肌肉组织中分离出旋毛虫幼虫。通过多重 PCR 将寄生虫鉴定为旋毛虫假型，对核大亚基核糖体 DNA 的扩展片段 V（ESV）区域的测序显示，在微卫星区域，存在适合古北界种群的多态性。这是在红鸢中首次发现旋毛虫假型，也是在意大利南部首次报告该寄生虫。寄生虫在留鸟中的分离证实，即使流行率较低，旋毛虫假型也存在于意大利的鸟类群中。
