增强型关节炎影响的人软骨细胞在聚合物三维（3D）基质中的 miRNA-140 表达。
Enhanced miRNA-140 expression of osteoarthritis-affected human chondrocytes cultured in a polymer based three-dimensional (3D) matrix.
机构信息
Edogawa Evolutionary Lab of Science, Edogawa Hospital Campus, 2-24-18, Higashi Koiwa, Edogawa, Tokyo 133-0052, Japan; Department of Orthopaedic Surgery, Edogawa Hospital, 2-24-18, Higashi Koiwa, Edogawa, Tokyo 133-0052, Japan.
Mebiol Inc., 1-25-8, Nakahara, Hiratsuka 254-0075, Kanagawa, Japan.
出版信息
Life Sci. 2021 Aug 1;278:119553. doi: 10.1016/j.lfs.2021.119553. Epub 2021 Apr 29.
AIMS
We have evaluated the potential of a three-dimensional (3D) thermoreversible gelation polymer (TGP) matrix in enhancing miRNA 140 expression (a biomarker correlating with homeostasis and cartilage regeneration) during the in vitro expansion of osteoarthritis (OA)-affected human chondrocytes.
MATERIALS AND METHODS
OA-chondrocytes were cultured in two-dimensional (2D) monolayer followed by culture in 3D-TGP. miRNA 140 expression levels in cell culture supernatant followed by expression in the cell lysate of both 2D and 3D-TGP cultures were analyzed.
KEY FINDINGS
The expression of miRNA 140 in cell culture supernatant from the 3D-TGP group was 0.001 to 0.002% that in 2D culture supernatant while in the cell lysate, miRNA 140 expression in the 3D-TGP was nearly 30-fold higher than that of 2D group.
SIGNIFICANCE
The 3D-TGP matrix allows enhanced expression of miRNA 140 in OA-affected human chondrocytes in vitro which after necessary validations can be applied in clinical transplantation to significantly improve the outcome.
目的
我们评估了三维（3D）热可逆凝胶聚合物（TGP）基质在体外扩增骨关节炎（OA）患者软骨细胞过程中增强 miRNA 140 表达（与内稳态和软骨再生相关的生物标志物）的潜力。
材料与方法
OA 软骨细胞先在二维（2D）单层中培养，然后在 3D-TGP 中培养。分析细胞培养上清液中 miRNA 140 的表达水平，然后分析 2D 和 3D-TGP 培养物细胞裂解物中的表达。
主要发现
3D-TGP 组细胞培养上清液中 miRNA 140 的表达水平为 0.001 至 0.002%，而在细胞裂解物中，3D-TGP 中的 miRNA 140 表达水平比 2D 组高近 30 倍。
意义
3D-TGP 基质可增强 OA 患者体外软骨细胞中 miRNA 140 的表达，经过必要的验证后可应用于临床移植，显著改善治疗效果。
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