Pharmacology and Toxicology Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran.
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
Eur J Pharmacol. 2021 Jul 15;903:174122. doi: 10.1016/j.ejphar.2021.174122. Epub 2021 Apr 28.
Immune-induced inflammation plays an important role both in aggravating and healing of post myocardial infarction (MI) injuries. Potent anti-inflammatory and local immunomodulatory activity of infliximab has been suggested to have modulating effects on immune responses after MI. The aim of the present study was to evaluate the efficacy of infliximab on hemodynamic responses and myocardial injuries following isoproterenol-induced myocardial infarction. Male Wistar rats, weighting 260 ± 20 g were assigned into ten groups (n = 6) of saline (normal saline), infliximab (7 mg/kg), isoproterenol (100 mg/kg for two consecutive days), and isoproterenol plus infliximab (30 min after the second injection of isoproterenol). The heart tissues and serums were analyzed 24, 48, 72, and 96 h post-MI and hemodynamic parameters, histopathological changes, malondialdehyde (MDA), Total antioxidant capacity (TAC), lactate dehydrogenase (LDH), and lactate levels were assessed in the respective groups. Infliximab partially improved hemodynamic depression in the first days after MI, but the heart became more suppressed later. A similar result also obtained at the MDA tissue levels but not serum levels. Anti-inflammatory effects of Infliximab may improve cardiac function and prevent heart tissue injury early after MI; however, it can worsen the condition later by inhibiting compensatory reactions such as cardiac remodeling and tissue repair.
免疫诱导的炎症在心肌梗死后（MI）损伤的加重和愈合中都起着重要作用。英夫利昔单抗具有强大的抗炎和局部免疫调节活性，被认为对 MI 后免疫反应具有调节作用。本研究旨在评估英夫利昔单抗对异丙肾上腺素诱导的心肌梗死后血流动力学反应和心肌损伤的疗效。雄性 Wistar 大鼠，体重 260±20g，分为盐水（生理盐水）、英夫利昔单抗（7mg/kg）、异丙肾上腺素（连续两天 100mg/kg）和异丙肾上腺素加英夫利昔单抗（第二次异丙肾上腺素注射后 30 分钟）十组（每组 n=6）。MI 后 24、48、72 和 96h 分析心脏组织和血清，评估血流动力学参数、组织病理学变化、丙二醛（MDA）、总抗氧化能力（TAC）、乳酸脱氢酶（LDH）和乳酸水平。英夫利昔单抗在 MI 后最初几天部分改善了血流动力学抑制，但后来心脏抑制更为严重。在 MDA 组织水平上也得到了类似的结果，但在血清水平上没有得到。英夫利昔单抗的抗炎作用可能在 MI 后早期改善心脏功能并预防心肌组织损伤；然而，它可能会通过抑制心脏重构和组织修复等代偿反应而使病情恶化。
