Department of Biochemistry, University of Madras, Guindy Campus, Chennai, Tamil Nadu, India.
Mol Cell Biochem. 2012 Aug;367(1-2):1-8. doi: 10.1007/s11010-012-1251-9. Epub 2012 Feb 15.
This study was designed to evaluate the anti-inflammatory and anti-apoptotic effects of the alcoholic extract of the berries of Crataegus oxyacantha (AEC), a medicinal herb, on isoproterenol-induced myocardial infarction (MI) in a rat model. Three groups of Wistar albino rats, each comprising six animals, were selected for this study. Group I rats served as control. Group II rats were given isoproterenol (85 mg/kg body weight) subcutaneously on 59th and 60th days. Group III rats were given AEC (0.5 ml/100 g body weight/day), orally on a daily basis for 60 days, and isoproterenol (85 mg/kg body weight, subcutaneously) was given on 59th and 60th days. On the 61st day, the animals were sacrificed, and marker enzymes like lactate dehydrogenase (LDH) and creatine kinase (CK) were estimated in serum. In the heart tissue sample, antioxidant status, lipid peroxidation and anti-inflammatory properties of AEC were determined. Isoproterenol significantly increased the release of LDH, CK in serum, decreased the antioxidant status in the heart along with an increase in lipid peroxidation. Nitritive stress and apoptosis were seen in isoproterenol-induced rat heart. Pre-treatment with the AEC for 60 days had a significant effect on all the above factors and maintained near normal status. The study confirms the protective effect of AEC against isoproterenol-induced inflammation and apoptosis-associated MI in rats.
本研究旨在评估山梨醇（Crataegus oxyacantha）浆果的醇提物（AEC）作为一种草药，对异丙肾上腺素诱导的大鼠心肌梗死（MI）的抗炎和抗凋亡作用。本研究选择了三组 Wistar 白化大鼠，每组包含六只动物。第 I 组大鼠作为对照。第 II 组大鼠在第 59 天和第 60 天皮下注射异丙肾上腺素（85mg/kg 体重）。第 III 组大鼠每天口服 AEC（0.5ml/100g 体重/天），连续 60 天，第 59 天和第 60 天皮下注射异丙肾上腺素（85mg/kg 体重）。第 61 天，处死动物，测定血清中乳酸脱氢酶（LDH）和肌酸激酶（CK）等标记酶。在心脏组织样本中，测定 AEC 的抗氧化状态、脂质过氧化和抗炎特性。异丙肾上腺素显著增加了血清中 LDH、CK 的释放，降低了心脏的抗氧化状态，同时增加了脂质过氧化。异丙肾上腺素诱导的大鼠心脏出现了营养性应激和细胞凋亡。用 AEC 预处理 60 天对所有上述因素均有显著影响，并使它们接近正常状态。该研究证实了 AEC 对异丙肾上腺素诱导的炎症和凋亡相关 MI 的保护作用。
