Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, PR China; Rural Energy & Environmental Protection Institute of Heilongjiang Academy of Agricultural Sciences, Key Laboratory Combining Farming & Animal Husbandry, Key Laboratory of Straw Energy Utilization, Harbin 150086, PR China.
Heilongjiang Academy of Agricultural Sciences, Harbin 150086, PR China.
Bioresour Technol. 2021 Aug;333:125204. doi: 10.1016/j.biortech.2021.125204. Epub 2021 Apr 24.
In this study, Anaerobic Digestion Model No. 1 (ADM1) were modified to simulate anaerobic digestion (AD) process of microcrystalline cellulose (MCC) and five lignocellulosic substrates, with the goal of predicting the hydrolysis rates of holocellulose fractions in environments with and without lignin inhibition. After model verification, the hydrolysis rate constant of MCC, i.e., the hydrolyzability of cellulose without lignin inhibition, was 3.227 d, while those of the holocellulose fractions of five lignocellulosic substrates (I_k) were in the range of 1.270 d to 3.364 d (average of 2.242 d), which demonstrated remarkable suppression of holocellulose hydrolysis by lignin. Lignin inhibition index (LII) was proposed as an indicator to intuitively quantify and characterize the lignin inhibitory strength in a specific substrate. A series of factors with the potential to affect the LII were analyzed sequentially. This study provides an advanced understanding of the participation and behavior of lignin in the AD process.
在本研究中，修改了 Anaerobic Digestion Model No. 1 (ADM1) 以模拟微晶纤维素 (MCC) 和五种木质纤维素基质的厌氧消化 (AD) 过程，目的是预测有和没有木质素抑制时的全纤维素分数的水解速率。在模型验证后，MCC 的水解速率常数，即没有木质素抑制时纤维素的可水解性，为 3.227 d，而五种木质纤维素基质的全纤维素分数的水解速率常数 (I_k) 在 1.270 d 至 3.364 d 范围内（平均值为 2.242 d），这表明木质素对全纤维素水解有显著的抑制作用。提出木质素抑制指数 (LII) 作为直观量化和表征特定基质中木质素抑制强度的指标。依次分析了一系列可能影响 LII 的因素。本研究提供了对木质素在 AD 过程中参与和行为的深入了解。
