Zhao Yeqi, Wang Tiancheng, Li Peng, Chen Jia, Nepovimova Eugenie, Long Miao, Wu Wenda, Kuca Kamil
College of Animal Science & Veterinary Medicine, Shenyang Agricultural University, Shenyang 110866, China.
Department of Chemistry, Faculty of Science, University of Hradec Kralove, 50003 Hradec Kralove, Czech Republic.
Ecotoxicol Environ Saf. 2021 Apr 29;218:112286. doi: 10.1016/j.ecoenv.2021.112286.
Aflatoxin B1(AFB1) widely exists in food and feed, which seriously endangers human and animal health. How to detoxify AFB1 is a research hotspot at present. This study attempts to use the Bacillus amyloliquefaciens B10, one of probiotics strain as the research object to ascertain whether it can alleviate the kidney injury induced by AFB1 in mice and its mechanism. Fifty-six mice were divided into four groups (control, AFB1, AFB1 + B10, and B10). The mice that received intragastric administration for 28 days were euthanised, and serum was collected for biochemical index detection with fresh kidney tissue taken for HE staining, TUNEL detection, and protein expression detection. Our results showed that the biochemical indices changed, significant pathological changes appeared, the number of apoptotic cells increased in the kidney tissue of the AFB1 group mice; the protein expressions of Nrf2, HO-1,AKT, P-AKT, and Bcl-2 in the AFB1 group were significantly decreased; the protein expressions of Keap-1, PTEN, Bax, Caspase-9, and Caspase-3 were significantly increased. After B. amyloliquefaciens B10 co-treatment, compared with the AFB1 group, the biochemical indices, pathological changes, and protein expressions were significantly reversed. The results indicated that B. amyloliquefaciens B10 can alleviate AFB1-induced kidney injury in mice.
黄曲霉毒素B1（AFB1）广泛存在于食品和饲料中，严重危害人类和动物健康。如何对AFB1进行解毒是目前的研究热点。本研究试图以益生菌菌株之一的解淀粉芽孢杆菌B10为研究对象，确定其是否能减轻AFB1诱导的小鼠肾损伤及其机制。将56只小鼠分为四组（对照组、AFB1组、AFB1 + B10组和B10组）。对接受灌胃28天的小鼠实施安乐死，收集血清用于生化指标检测，并取新鲜肾组织进行苏木精-伊红（HE）染色、TUNEL检测和蛋白表达检测。我们的结果显示，AFB1组小鼠的生化指标发生变化，出现明显的病理变化，肾组织中凋亡细胞数量增加；AFB1组中Nrf2、HO-1、AKT、磷酸化AKT（P-AKT）和Bcl-2的蛋白表达显著降低；Keap-1、PTEN、Bax、Caspase-9和Caspase-3的蛋白表达显著增加。解淀粉芽孢杆菌B10联合处理后，与AFB1组相比，生化指标、病理变化和蛋白表达均得到显著逆转。结果表明，解淀粉芽孢杆菌B10可减轻AFB1诱导的小鼠肾损伤。
