基于膜保护共价有机框架纤维的牛奶中 17β-雌二醇的直接浸取固相微萃取
Membrane-protected covalent organic framework fiber for direct immersion solid-phase microextraction of 17beta-estradiol in milk.
机构信息
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Institute of Analytical Food Safety, School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
State Key Laboratory of Food Science and Technology, School of Food Science and Technology, Jiangnan University, Wuxi 214122, China.
出版信息
Food Chem. 2021 Oct 15;359:129816. doi: 10.1016/j.foodchem.2021.129816. Epub 2021 Apr 20.
17beta-estradiol (E2) could accumulate in human body through milk and cause various diseases by interfering with the endocrine system. Herein, we coated stainless steel wire with covalent organic framework LZU1 (COF-LZU1) and Nafion protected by dialysis membrane for direct immersion solid phase microextraction (DI-SPME) and coupled with gas chromatography-flame ionization detection (GC-FID) for the detection of trace E2 in milk samples. With dialysis membrane protection, the stability of SPME fiber was improved and the extraction efficiency was only reduced by 7% after repeated use of 160 times. The extraction efficiency of E2 with the home-made fiber COF-LZU1 was 22.1, 8.4, 3.6 times higher than that of bare stainless steel wire, PDMS/DVB and PDMS, respectively. The method had been successfully applied to milk samples, and the relative recoveries were between 77.27% and 108.26%. It can provide an effective and general method for the pretreatment of complex matrix samples.
17β-雌二醇（E2）可通过乳汁在人体内蓄积，并通过干扰内分泌系统引起各种疾病。在此，我们通过在透析膜保护下的共价有机框架 LZU1（COF-LZU1）和 Nafion 对不锈钢丝进行涂层，用于直接浸入式固相微萃取（DI-SPME），并与气相色谱-火焰离子化检测（GC-FID）耦合，用于检测牛奶样品中的痕量 E2。通过透析膜保护，SPME 纤维的稳定性得到提高，并且在重复使用 160 次后，萃取效率仅降低了 7%。自制纤维 COF-LZU1 对 E2 的萃取效率分别比裸不锈钢丝、PDMS/DVB 和 PDMS 高 22.1、8.4 和 3.6 倍。该方法已成功应用于牛奶样品，并具有 77.27%至 108.26%的相对回收率。它可以为复杂基质样品的预处理提供一种有效且通用的方法。
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