Noncommunicable Diseases Research Center, Bam University of Medical Sciences, Bam, Iran.
School of Medicine, Bam University of Medical Sciences, Bam, Iran.
Bioprocess Biosyst Eng. 2021 Sep;44(9):1957-1964. doi: 10.1007/s00449-021-02576-w. Epub 2021 May 1.
The aim of this study was cost-effective and greener synthesis of barium carbonate (BaCO or witherite) nanoparticles with economic importance, and to evaluate their therapeutic potentials and biocompatibility with immune cells. Barium carbonate nanoparticles were biosynthesized using black elderberry extract in one step with non-toxic precursors and simple laboratory conditions; their morphologies and specific structures were analyzed using field emission scanning electron microscopy with energy dispersive X-ray spectroscopy (FESEM-EDX). The therapeutic capabilities of these nanoparticles on the immune cells of murine macrophages J774 and promastigotes Leishmania tropica were evaluated. BaCO nanoparticles with IC = 46.6 µg/mL were more effective than negative control and glucantium (positive control) in reducing promastigotes (P < 0.01). Additionally, these nanoparticles with a high value of cytotoxicity concentration 50% (CC) were less toxic to macrophage cells than glucantime; however, they were significantly different at high concentrations compared to the negative control.
本研究旨在通过经济且环保的方法，使用具有经济重要性的黑接骨木提取物，在无毒前体和简单的实验室条件下一步合成碳酸钡（BaCO 或菱锶矿）纳米粒子，并使用场发射扫描电子显微镜结合能谱分析（FESEM-EDX）对其形态和特定结构进行分析。评估了这些纳米粒子对鼠源巨噬细胞 J774 和利什曼原虫前鞭毛体的免疫细胞的治疗潜力和生物相容性。IC=46.6µg/mL 的 BaCO 纳米粒子在减少前鞭毛体方面比阴性对照和葡萄糖胺（阳性对照）更有效（P<0.01）。此外，这些具有高细胞毒性浓度 50%（CC）的纳米粒子对巨噬细胞的毒性低于葡萄糖胺；然而，与阴性对照相比，在高浓度时差异显著。
