Department of Clinical Laboratory Medicine, The First Medical Center, Chinese Peoples' Liberation Army General Hospital, Beijing, P.R. China.
School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi'an, P.R. China.
Biofouling. 2021 Mar;37(3):327-340. doi: 10.1080/08927014.2021.1914021. Epub 2021 May 3.
Biofilm formation by involves formation of an extracellular matrix; however, the identity of the constituents responsible for the structure of biofilms fabricated by different clinical strains is largely unclear. Here, biofilms produced by 24 clinical isolates of were characterized. The optimal medium for was selected, and the biofilm-forming capacity was assessed. Extracelullar polymeric substances (EPS) contributing to biofilm robustness were determined by evaluating the susceptibility of biofilms to EPS-degrading agents using field emission scanning electron microscopy and confocal laser scanning microscopy. Biofilm formation by the clinical isolates of was augmented by glucose supplementation. Further, extracellular DNA (eDNA), proteins, and polysaccharides were present in the 24 clinical isolates. Proteins and polysaccharides were the most common components within the biofilms, whereas the eDNA content was marginal in biofilm formation. Therefore, proteins and polysaccharides within biofilms may be used as the primary targets for developing eradication strategies to prevent biofilm formation.
生物膜的形成涉及细胞外基质的形成；然而，不同临床菌株所形成的生物膜结构的组成成分在很大程度上尚不清楚。在这里，对 24 株临床分离株的生物膜进行了表征。选择了 的最佳培养基，并评估了其生物膜形成能力。通过使用场发射扫描电子显微镜和共聚焦激光扫描显微镜评估生物膜对 EPS 降解剂的敏感性，确定了有助于生物膜坚固性的细胞外聚合物质 (EPS)。葡萄糖的补充促进了临床分离株的生物膜形成。此外，24 株临床分离株中存在胞外 DNA（eDNA）、蛋白质和多糖。在 生物膜中，蛋白质和多糖是最常见的成分，而 eDNA 在生物膜形成中的含量很少。因此，生物膜中的蛋白质和多糖可以作为开发根除策略的主要靶点，以防止 生物膜形成。
