Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China; State Key Laboratory of Transducer Technology, Chinese Academy of Sciences, Beijing, 100080, China.
Key Laboratory of Combinatorial Biosynthesis and Drug Discovery, Ministry of Education, Hubei Province Engineering and Technology Research Center for Fluorinated Pharmaceuticals, Wuhan University School of Pharmaceutical Sciences, Wuhan, 430071, China.
Talanta. 2021 Aug 1;230:122330. doi: 10.1016/j.talanta.2021.122330. Epub 2021 Mar 23.
Column technology is an important part in capillary electrochromatographic science. Developing novel stationary phase with high separation efficiency and high loading capacity is an essential work. In this work, a novel spherical vinyl-functionalized covalent-organic framework (COF-V) was synthesized at room temperature and firstly employed as stationary phase for CEC-MS analysis. The COF-V based CEC column was characterized by scanning electron microscopy, X-ray diffraction and Fourier transform infrared spectroscopy. The results proved the successful modification of COF-V. The COF-V based column possesses the advantages like strong electroosmotic flow, high separation efficiency and high loading capacity. The CEC column showed powerful separation selectivity to several kinds of compounds, and the highest column efficiency (theoretical plates, N) was over 1.4 × 10 plates·m for methylbenzene. Besides, the COF-V modified column exhibited excellent repeatability and stability. The relative standard deviations (RSDs) of retention times for intra-day (n = 5), inter-day (n = 3) runs and column-to-column (n = 3) were all less than 2.1%. Hence, the COF-V modified column was successfully applied in CEC-MS for determination of antiepileptic drug, triazine herbicides and active ingredients in traditional Chinese medicine.
柱技术是毛细管电色谱科学的重要组成部分。开发具有高分离效率和高载量的新型固定相是一项必不可少的工作。在这项工作中，我们在室温下合成了一种新型的球形乙烯基功能化共价有机骨架（COF-V），并首次将其用作 CEC-MS 分析的固定相。通过扫描电子显微镜、X 射线衍射和傅里叶变换红外光谱对 COF-V 基 CEC 柱进行了表征。结果证明了 COF-V 的成功修饰。COF-V 基柱具有强电动流、高分离效率和高载量的优点。该 CEC 柱对多种化合物表现出强大的分离选择性，最高柱效（理论塔板数，N）超过 1.4×10 块·m-1 用于甲苯。此外，COF-V 修饰柱表现出优异的重现性和稳定性。日内（n=5）、日间（n=3）和柱间（n=3）运行的保留时间相对标准偏差（RSD）均小于 2.1%。因此，COF-V 修饰柱成功应用于 CEC-MS 测定抗癫痫药物、三嗪类除草剂和中药有效成分。
