Bie Zijun, Chen Yang
Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233030, China; School of Pharmacy, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233000, China.
Department of Chemistry, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233030, China; School of Pharmacy, Bengbu Medical University, 2600 Donghai Avenue, Bengbu, 233000, China.
Talanta. 2021 Aug 1;230:122338. doi: 10.1016/j.talanta.2021.122338. Epub 2021 Mar 23.
Interferons are important biomolecules in human immune system. Cytokine interferon alpha (IFN-α), a type I interferon, is one of the major components of the innate immune response involved in autoimmune diseases. Thus, the analysis of interferons is of great importance for both biological and pharmaceutical purposes. In this work, an IFN-α specific plastic antibody is prepared via boronate affinity oriented surface imprinting. By combing with the magnetic nanoparticles, the imprinted material exhibits several advantages, including strong affinity (K: 75.2 nM), high specificity (cross reactivity<25%), excellent efficiency (imprinting efficiency: 44.1%), tolerance to interferences, and easy manipulation. By employing the prepared imprinted material as sorbent for selective enrichment of IFN-α, a good linearity is achieved in the range of 50 ng/mL-10 μg/mL, and the detection and quantifcation limits are 10 ng/mL and 50 ng/mL respectively. The recoveries of this approach are found within 75.8%-82.2% with relative standard deviations of 6.4-9.7%. Furthermore, the IFN-α in spiked human serum is analyzed with acceptable reliability (recovery: 77.3%, RSD: 7.9%). Because of these highly desirable properties, the IFN-α specific plastic antibody can find more applications in medical and pharmaceutical industry.
干扰素是人体免疫系统中的重要生物分子。细胞因子干扰素α（IFN-α）属于I型干扰素，是参与自身免疫性疾病的先天免疫反应的主要成分之一。因此，干扰素的分析对于生物学和制药目的都非常重要。在这项工作中，通过硼酸酯亲和导向表面印迹法制备了一种IFN-α特异性塑料抗体。通过与磁性纳米颗粒结合，印迹材料具有多种优点，包括强亲和力（K：(75.2 nM)）、高特异性（交叉反应性<25%）、优异的效率（印迹效率：44.1%）、抗干扰能力以及易于操作。通过使用制备的印迹材料作为吸附剂选择性富集IFN-α，在(50 ng/mL - 10 μg/mL)范围内实现了良好的线性，检测限和定量限分别为(10 ng/mL)和(50 ng/mL)。该方法的回收率在(75.8% - 82.2%)之间，相对标准偏差为(6.4 - 9.7%)。此外，对加标的人血清中的IFN-α进行分析，可靠性良好（回收率：77.3%，相对标准偏差：7.9%）。由于这些非常理想的特性，IFN-α特异性塑料抗体在医疗和制药行业中可以有更多应用。
