School of Pharmacy, Anhui University of Chinese Medicine, Hefei, 230012, China.
Anhui Province Key Laboratory of Pharmaceutical Preparation Technology and Application, Hefei, Anhui, 230012, China.
Curr Protein Pept Sci. 2024;25(4):286-306. doi: 10.2174/0113892037277894231208065403.
The separation of proteins in biological samples plays an essential role in the development of disease detection, drug discovery, and biological analysis. Protein imprinted polymers (PIPs) serve as a tool to capture target proteins specifically and selectively from complex media for separation purposes. Whereas conventional molecularly imprinted polymer is time-consuming in terms of incubation studies and solvent removal, magnetic particles are introduced using their magnetic properties for sedimentation and separation, resulting in saving extraction and centrifugation steps. Magnetic protein imprinted polymers (MPIPs), which combine molecularly imprinting materials with magnetic properties, have emerged as a new area of research hotspot. This review provides an overview of MPIPs for proteins, including synthesis, preparation strategies, and applications. Moreover, it also looks forward to the future directions for research in this emerging field.
蛋白质在生物样本中的分离在疾病检测、药物发现和生物分析的发展中起着至关重要的作用。蛋白质印迹聚合物（PIP）是一种从复杂介质中特异性和选择性捕获靶蛋白的工具，用于分离目的。而传统的分子印迹聚合物在孵育研究和溶剂去除方面耗时较长，而磁性颗粒则利用其磁性特性进行沉降和分离，从而节省了提取和离心步骤。磁性蛋白质印迹聚合物（MPIP）将分子印迹材料与磁性相结合，已成为一个新的研究热点领域。本文综述了蛋白质 MPIP 的合成、制备策略及应用等方面的研究进展，并对该新兴领域的未来研究方向进行了展望。
