Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Xiamen, Fujian, China.
College of Biological Science and Engineering, Fuzhou University, Fuzhou, Fujian, 350116, China.
Talanta. 2021 Aug 1;230:122364. doi: 10.1016/j.talanta.2021.122364. Epub 2021 Mar 26.
To meet the increasing need for point-of-care testing (POCT), simple and portable readout strategies would be highly desirable. Thermometer with high accuracy and straightforward readout is an ideal tool for the development of new POCT methods. The exploration of new thermometer-based detection methods is of great significance. In this study, a simple biosensor for glucose based on the photothermal effect of gold nanorods using a simple thermometer as readout has been developed. In the presence of glucose oxidase, glucose can react with the dissolved oxygen to produce HO. With the help of Fe, HO can etch gold nanorods (AuNRs) to different aspect ratios. The decrease of the aspect ratio of AuNRs leads to the blue-shift of the localized surface plasmon resonance peak, resulting in a decrease of photothermal effect in the near-infrared regions and the temperature of the system decreased. The change of the temperature has a linear relationship with the logarithm of glucose concentration in the range of 1.0-10.0 mM with a detection limit of 0.8 mM. The proposed method possesses a bias offset of -0.03 mM for glucose detection compared to the hospital method. Since many enzymatic reactions can produce HO, the principle can be modified to detect different targets by simply change of the enzyme used.
为了满足即时检测（POCT）日益增长的需求，简单且便携的读出策略将是非常需要的。具有高精度和简单读出的温度计是开发新的 POCT 方法的理想工具。探索基于温度计的新型检测方法具有重要意义。在这项研究中，我们开发了一种基于金纳米棒光热效应的简单葡萄糖生物传感器，使用简单的温度计作为读出。在葡萄糖氧化酶存在的情况下，葡萄糖可以与溶解氧反应生成 HO。在 Fe 的帮助下，HO 可以刻蚀金纳米棒（AuNRs）到不同的纵横比。AuNRs 的纵横比降低会导致局域表面等离激元共振峰蓝移，导致近红外区域的光热效应降低，系统温度降低。温度的变化与葡萄糖浓度在 1.0-10.0 mM 范围内呈对数线性关系，检测限为 0.8 mM。与医院方法相比，该方法检测葡萄糖的偏置偏移为-0.03 mM。由于许多酶反应都可以产生 HO，因此只需改变所用的酶，就可以修改该原理来检测不同的靶标。
