Pu Jiamei, Liu Mingbin, Li Hongbo, Liao Zhigang, Zhao Weihua, Wang Suqin, Zhang Yun, Yu Ruqin
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China.
Key Laboratory of Functional Small Organic Molecule, Ministry of Education, College of Chemistry and Chemical Engineering, Jiangxi Normal University, Nanchang, 330022, PR China.
Talanta. 2021 Aug 1;230:122158. doi: 10.1016/j.talanta.2021.122158. Epub 2021 Feb 19.
MicroRNAs (miRNAs) play a significant role in diverse biological processes. The abnormal expression of miRNAs is related to the development of cancers and various diseases. It is of great importance to sensitively and accurately detect miRNAs for early disease diagnosis and treatment. Here, a new fluorescence strategy was initially proposed for the enzyme-free sensing of let-7a by combining the strand displacement reaction (SDR) with the hybridization chain reaction (HCR). The sensor was successfully applied to the detection of the let-7a gene with a wide linear range from 25 pM to 250 nM and a limit of detection (LOD) of 9.01 pM. The fluorescence intensity has a good linear relationship with the logarithm of the target concentration. In addition, the biosensor allowed for the highly sensitive detection of the target genes even in complex human serum samples. With simple operation yet improved detection capability for let-7a, the developed fluorescent biosensor thus shows great potential for early clinical diagnosis as well as biological research.
微小RNA（miRNA）在多种生物过程中发挥着重要作用。miRNA的异常表达与癌症及各种疾病的发生发展相关。灵敏且准确地检测miRNA对于疾病的早期诊断和治疗至关重要。在此，首次提出了一种新的荧光策略，通过将链置换反应（SDR）与杂交链式反应（HCR）相结合，实现对let-7a的无酶传感。该传感器成功应用于let-7a基因的检测，线性范围宽，为25 pM至250 nM，检测限（LOD）为9.01 pM。荧光强度与目标浓度的对数具有良好的线性关系。此外，该生物传感器即使在复杂的人血清样本中也能实现对目标基因的高灵敏检测。所开发的荧光生物传感器操作简单，但对let-7a的检测能力有所提高，因此在早期临床诊断以及生物学研究中显示出巨大潜力。
