Department of Health Sciences and Technology (D-HEST), ETH Zurich, Institute of Translational Medicine (ITM), Zurich, Switzerland; Swiss Institute of Bioinformatics (SIB), Lausanne, Switzerland.
Department of Health Sciences and Technology (D-HEST), ETH Zurich, Institute of Translational Medicine (ITM), Zurich, Switzerland; Swiss Institute of Bioinformatics (SIB), Lausanne, Switzerland.
Adv Virus Res. 2021;109:105-134. doi: 10.1016/bs.aivir.2021.03.002. Epub 2021 Apr 12.
The cellular surfaceome and its residing extracellularly exposed proteins are involved in a multitude of molecular signaling processes across the viral infection cycle. Successful viral propagation, including viral entry, immune evasion, virion release and viral spread rely on dynamic molecular interactions with the surfaceome. Decoding of these viral-host surfaceome interactions using advanced technologies enabled the discovery of fundamental new functional insights into cellular and viral biology. In this review, we highlight recently developed experimental strategies, with a focus on spatial proteotyping technologies, aiding in the rational design of theranostic strategies to combat viral infections.
细胞表面组及其驻留的细胞外暴露蛋白参与病毒感染周期中的多种分子信号转导过程。成功的病毒繁殖，包括病毒进入、免疫逃逸、病毒释放和病毒传播，都依赖于与表面组的动态分子相互作用。使用先进技术对这些病毒-宿主表面组相互作用进行解码，为细胞和病毒生物学的基本新功能见解提供了依据。在这篇综述中，我们重点介绍了最近开发的实验策略，特别是空间蛋白质组学技术，这有助于合理设计治疗病毒感染的治疗策略。
