Rauth G, Schumacher G, Buckel P, Müller-Esterl W
Abteilung für Klinische Chemie, Chirurgische Klinik Innenstadt der Universität, München, Federal Republic of Germany.
Protein Seq Data Anal. 1988;1(4):251-7.
A clone coding for the precursor form of C1 inhibitor has been isolated from a human liver cDNA library constructed in lambda gt11. This clone contains a cDNA insert of 1777 nucleotides, which includes 63 nucleotides coding for a signal sequence of 21 amino acids, 1434 nucleotides coding for the mature protein of 478 amino acids, a stop codon of TGA, and 265 nucleotides of the 3'-noncoding sequence followed by a poly(A) tail of 12 nucleotides. The predicted amino acid sequence of mature C1 inhibitor contains a unique region (positions 43 to 97) of 14 tandemly repeated copies of the tetrapeptide Gln-Pro-Thr-Thr and variants thereof. The carboxy-terminal sequence (positions 98 to 478) comprises the typical structural elements of a serine proteinase inhibitor (serpin). These findings indicate that C1 inhibitor is unique among the known members of the serpin superfamily due to the presence of an extra-domain of highly repetitive structure located at the amino-terminus of the inhibitor.
已从构建于λgt11中的人肝cDNA文库中分离出编码C1抑制剂前体形式的克隆。该克隆包含一个1777个核苷酸的cDNA插入片段，其中包括63个编码21个氨基酸信号序列的核苷酸、1434个编码478个氨基酸成熟蛋白的核苷酸、一个TGA终止密码子，以及265个3'非编码序列核苷酸，后面跟着一个12个核苷酸的聚腺苷酸尾巴。成熟C1抑制剂的预测氨基酸序列包含一个由14个串联重复的四肽Gln-Pro-Thr-Thr及其变体组成的独特区域（第43至97位）。羧基末端序列（第98至478位）包含丝氨酸蛋白酶抑制剂（丝氨酸蛋白酶抑制剂超家族）的典型结构元件。这些发现表明，C1抑制剂在丝氨酸蛋白酶抑制剂超家族的已知成员中是独特的，因为在抑制剂的氨基末端存在一个高度重复结构的额外结构域。
