抗体对协同反应的催化作用：克莱森重排反应
Catalysis of concerted reactions by antibodies: the Claisen rearrangement.
作者信息
Hilvert D, Carpenter S H, Nared K D, Auditor M T
机构信息
Department of Molecular Biology, Research Institute of Scripps Clinic, La Jolla, CA 92037.
出版信息
Proc Natl Acad Sci U S A. 1988 Jul;85(14):4953-5. doi: 10.1073/pnas.85.14.4953.
Abstract
Monoclonal antibodies were prepared against a transition state analog inhibitor of chorismate mutase (EC 5.4.99.5). One of the antibodies catalyzes the rearrangement of chorismate to prephenate with rate accelerations of more than 2 orders of magnitude compared to the uncatalyzed reaction. Saturation kinetics were observed, and at 25 degrees C the values of kcat and Km were 1.2 X 10(-3) s-1 and 5.1 X 10(-5) M respectively. The transition state analog was shown to be a competitive inhibitor of the reaction with Ki equal to 0.6 microM. These results demonstrate the feasibility of using rationally designed immunogens to generate antibodies that catalyze concerted reactions.
摘要
制备了针对分支酸变位酶（EC 5.4.99.5）过渡态类似物抑制剂的单克隆抗体。其中一种抗体催化分支酸重排为预苯酸，与未催化反应相比，速率加速超过2个数量级。观察到饱和动力学，在25℃时，kcat和Km值分别为1.2×10⁻³ s⁻¹和5.1×10⁻⁵ M。过渡态类似物被证明是该反应的竞争性抑制剂，Ki等于0.6 microM。这些结果证明了使用合理设计的免疫原产生催化协同反应的抗体的可行性。
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