Liu Lihong, Wen Lianpu, Gao Chuanzhou, Piao Hua, Zhao Hui, Yu Deqin, Zhu Liang, Li Shuzhuang
Department of Physiology, College of Basic Medical Sciences, Dalian Medical University, Dalian, China.
Central Laboratory, Dalian Medical University, Dalian, China.
Front Physiol. 2021 Apr 15;12:649554. doi: 10.3389/fphys.2021.649554. eCollection 2021.
Mechanical trauma can (MT) cause secondary injury, such as cardiomyocyte apoptosis and cardiac dysfunction has been reported. However, the effects of mechanical trauma on gastrointestinal tract is unclear. This study aims to observe the main location and time of gastrointestinal tract injury caused by non-directional trauma and explain the reason of the increase of LPS in blood caused by mechanical injury. Morphological changes in the stomach, ileum and cecum at different time points after MT were observed in this experiment. The results reveal that the injury to the cecal mucosa in the rats was more obvious than that in the ileum and the stomach. The cecal epithelial cell junction was significantly widened at 20 min after MT, and the plasma LPS and D-lactic acid concentrations increased significantly at the same time point. In addition, some bacterial structures in the widened intercellular space and near the capillary wall of the cecal mucosa were detected at 12 h after MT. This finding suggests that the main reason for the increase in LPS in plasma after MT is cecal mucosal injury. This study is important for the early intervention of the gastrointestinal tract to prevent secondary injury after MT.
机械创伤（MT）可导致继发性损伤，如已报道的心肌细胞凋亡和心脏功能障碍。然而，机械创伤对胃肠道的影响尚不清楚。本研究旨在观察非定向创伤所致胃肠道损伤的主要部位和时间，并解释机械损伤导致血液中脂多糖（LPS）升高的原因。本实验观察了MT后不同时间点胃、回肠和盲肠的形态学变化。结果显示，大鼠盲肠黏膜损伤比回肠和胃更明显。MT后20分钟，盲肠上皮细胞连接显著增宽，同时血浆LPS和D-乳酸浓度显著升高。此外，MT后12小时在盲肠黏膜增宽的细胞间隙和毛细血管壁附近检测到一些细菌结构。这一发现表明，MT后血浆中LPS升高的主要原因是盲肠黏膜损伤。本研究对于胃肠道的早期干预以预防MT后的继发性损伤具有重要意义。
