Feye Kristina M, Dittoe Dana K, Rubinelli Peter M, Olson Elena G, Ricke Steven C
Center for Food Safety, Department of Food Science, University of Arkansas, Fayetteville, AR, United States.
Meat Science and Animal Biologics Discovery, Animal and Dairy Sciences, University of Wisconsin-Madison, Madison, WI, United States.
Front Microbiol. 2021 Apr 15;12:645301. doi: 10.3389/fmicb.2021.645301. eCollection 2021.
Reading is an ongoing public health issue in the turkey industry, leading to significant morbidity in humans in the United States. Pre-harvest intervention strategies that contribute to the reduction of foodborne pathogens in food animals, such as the yeast fermentation metabolites of Original XPC (XPC), may become the key to multi-hurdle farm to fork strategies. Therefore, we developed an anaerobic turkey cecal model to assess the effects of XPC on the ceca of commercial finisher tom turkeys fed diets void of XPC and antibiotics. Using the turkey cecal culture method, ceca were tested with and without XPC for their anti- Reading and the previously defined anti-Typhimurium (ST97) effects. Ultimately, the anti- effects were independent of serovar ( > 0.05). At 0 h post inoculation (hpi), levels were equivalent between treatments at 7.3 Log CFU/mL, and at 24 hpi, counts in XPC were reduced by 5 Log CFU/mL, which was 2.1 Log lower than the control ( < 0.05). No differences in serovar prevalence existed ( > 0.05), with a 92% reduction in positive XPC-treated ceca cultures by 48 hpi ( < 0.05). To evaluate changes to the microbiota independent of the immune response, the 16S rDNA was sequenced using the Illumina MiSeq platform. Data indicated a profound effect of time and treatment for the reduction of irrespective of serovar. XPC sustained diversity metrics compared to the control, demonstrating a reduction in diversity over time ( < 0.05).
在火鸡养殖行业中，阅读是一个持续存在的公共卫生问题，在美国导致人类出现显著发病率。收获前的干预策略有助于减少食用动物体内的食源性病原体，例如原始XPC（XPC）的酵母发酵代谢产物，可能成为从农场到餐桌多环节策略的关键。因此，我们开发了一种厌氧火鸡盲肠模型，以评估XPC对饲喂不含XPC和抗生素日粮的商品育肥期公火鸡盲肠的影响。使用火鸡盲肠培养方法，对添加和不添加XPC的盲肠进行抗阅读和先前定义的抗鼠伤寒（ST97）效果测试。最终，抗效果与血清型无关（>0.05）。接种后0小时（hpi），各处理组的水平相当，为7.3 Log CFU/mL，在24 hpi时，XPC组的计数减少了5 Log CFU/mL，比对照组低2.1 Log（<0.05）。血清型流行率没有差异（>0.05），到48 hpi时，XPC处理的盲肠培养物中阳性减少了92%（<0.05）。为了评估独立于免疫反应的微生物群变化，使用Illumina MiSeq平台对16S rDNA进行测序。数据表明，无论血清型如何，时间和处理对减少的影响都很显著。与对照组相比，XPC维持了多样性指标，表明随着时间的推移多样性降低（<0.05）。 （注：原文中“anti- Reading”表述似乎有误，可能影响准确理解，暂按此翻译）
