Lee Do-Geun, Park Ji-Eun, Kim Mi-Ju, Kim Hyun-Joong, Kim Hae-Yeong
Institute of Life Sciences & Resources and Department of Food Science & Biotechnology, Kyung Hee University, Yongin, 17104 Republic of Korea.
Department of Food Engineering, Mokpo National University, Muan, 58554 Republic of Korea.
Food Sci Biotechnol. 2021 Mar 13;30(4):565-570. doi: 10.1007/s10068-021-00882-3. eCollection 2021 Apr.
As of 2020, 11 GM canola events have been authorized as food for humans in Korea. However, there are no simultaneous multiplex detection methods for 3 GM canola events (DP-073496-4, MON88302, and MS11). Thus, we established the multiplex polymerase chain reaction (PCR) method coupled with capillary electrophoresis to detect 3 GM canola events. To verify the specificity of event-specific primers, various GM crops of 3 GM soybean events, 6 GM maize events, 2 GM cotton events and 11 GM canola events were prepared. The limit of detection of the developed multiplex PCR was approximately 0.0125% for 3 GM canola events. Certified GM canola and stacked events were analyzed to validate the developed multiplex PCR. This study focuses on establishing multiplex PCR coupled with capillary electrophoresis for newly approved GM canola events and contributes to efficient monitoring GM canola samples in Korea.
截至2020年，韩国已批准11种转基因油菜事件作为人类食品。然而，对于3种转基因油菜事件（DP-073496-4、MON88302和MS11），尚无同步多重检测方法。因此，我们建立了结合毛细管电泳的多重聚合酶链反应（PCR）方法来检测3种转基因油菜事件。为验证事件特异性引物的特异性，准备了3种转基因大豆事件、6种转基因玉米事件、2种转基因棉花事件和11种转基因油菜事件的各种转基因作物。所开发的多重PCR对3种转基因油菜事件的检测限约为0.0125%。对认证的转基因油菜和叠加事件进行分析，以验证所开发的多重PCR。本研究重点在于为新批准的转基因油菜事件建立结合毛细管电泳的多重PCR，有助于韩国对转基因油菜样品进行高效监测。
