Shanghai Animal Disease Control Center, 855 Hongjin Road, Shanghai 201103, China.
Tianjin University of Traditional Chinese Medicine, Tianjin 301617, China.
Biomed Res Int. 2021 Apr 15;2021:5588855. doi: 10.1155/2021/5588855. eCollection 2021.
To construct a protein fingerprint database of (), thus improving its clinical diagnosis efficiency. A total of 15 standard strains were collected to establish a protein fingerprint database of using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. In addition, 18 clinical isolates were used to compare the identification results of the self-built protein fingerprint database, PCR detection, and basic database. Only one strain was identified in the original VITEK-MS system database, while the self-made protein fingerprint database of was 100% accurate for the detection of 18 clinical isolate strains. The protein fingerprint database of built by our laboratory is suitable for rapid clinical diagnosis of , due to its high accuracy, efficiency, and strong specificity.
构建金黄色葡萄球菌的蛋白质指纹图谱数据库，以提高其临床诊断效率。本研究采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术，收集 15 株标准菌株，建立金黄色葡萄球菌蛋白质指纹图谱数据库，并考察不同培养基和培养时间对蛋白质指纹图谱质量和鉴定结果的影响。结果表明，胰蛋白胨大豆琼脂（TSA）和胰蛋白胨大豆肉汤（TSB）培养基以及不同的孵育时间对蛋白质图谱的特征峰没有显著影响。此外，用自建的蛋白质指纹图谱数据库与传统的 VITEK-MS 系统数据库、PCR 检测及基础数据库对 18 株临床分离株进行鉴定结果比较，传统 VITEK-MS 系统数据库仅能鉴定出 1 株，而自建的金黄色葡萄球菌蛋白质指纹图谱数据库对 18 株临床分离株的检测准确率为 100%。本实验室构建的金黄色葡萄球菌蛋白质指纹图谱数据库具有准确率高、效率高、特异性强等优点，适用于金黄色葡萄球菌的快速临床诊断。
