Cardozo Gustavo Gonçalves, Lopes Karynne Grutter, Bottino Daniel Alexandre, Souza Maria das Graças Coelho de, Bouskela Eliete, Farinatti Paulo, Brandão de Oliveira Ricardo
Graduate Program in Exercise and Sport Sciences, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Laboratory of Physical Activity and Health Promotion, Institute of Physical Education and Sports, Rio de Janeiro State University, Rio de Janeiro, Brazil.
Contemp Clin Trials Commun. 2021 Mar 25;22:100740. doi: 10.1016/j.conctc.2021.100740. eCollection 2021 Jun.
Gains in muscle mass and strength have been documented in exercise training with blood flow restriction (BFR). However, the impact of retrograde blood flow during BFR training on vascular health remains unclear. The present study designed a protocol to evaluate the acute effects of exercise performed with different levels of BFR on vascular reactivity and biomarkers of endothelial function, oxidative stress, and muscle hypertrophy in young and older individuals.
Sixty-eight physically inactive eutrophic men [34 young (18-25-yrs old) and 34 elderly (≥65-yrs old)] will be included in the study. Subjects will undergo three experimental protocols: a) control (ExCON) - handgrip exercise with intensity of 30% of the maximum voluntary contraction (MVC); b) blood flow restriction (ExBFR) - handgrip exercise with a resistance of 30% of the MVC with low level of BFR [80% of arterial occlusion pressure at rest (rAOP)]; and c) arterial occlusion pressure (ExAOP) - handgrip exercise with a resistance of 30% of the MVC with high level of BFR (120% of rAOP). Primary outcomes will be: a) vascular reactivity assessed by venous occlusion plethysmography; b) endothelial function (nitric oxide and apoptotic endothelial micro particles; c) oxidative stress (thiobarbituric acid reactive substances). Growth hormone and lactate concentration will be measured as secondary outcomes reflecting the hypertrophic drive and metabolic stress, respectively.
The findings of the present study may help to elucidate the age-related impacts of BFR training on the vascular health.
已有文献记载，在采用血流限制（BFR）的运动训练中，肌肉质量和力量会有所增加。然而，BFR训练期间逆行血流对血管健康的影响仍不明确。本研究设计了一项方案，以评估不同程度BFR运动对年轻和老年个体血管反应性以及内皮功能、氧化应激和肌肉肥大生物标志物的急性影响。
本研究将纳入68名身体不活跃的营养良好男性[34名年轻人（18 - 25岁）和34名老年人（≥65岁）]。受试者将接受三种实验方案：a）对照组（ExCON）——以最大自主收缩（MVC）强度的30%进行握力运动；b）血流限制组（ExBFR）——以MVC强度的30%进行握力运动，采用低水平BFR[静息时动脉闭塞压（rAOP）的80%]；c）动脉闭塞压组（ExAOP）——以MVC强度的30%进行握力运动，采用高水平BFR（rAOP的120%）。主要结果将包括：a）通过静脉闭塞体积描记法评估血管反应性；b）内皮功能（一氧化氮和凋亡内皮微粒）；c）氧化应激（硫代巴比妥酸反应性物质）。生长激素和乳酸浓度将作为次要结果进行测量，分别反映肥大驱动和代谢应激。
本研究结果可能有助于阐明BFR训练对血管健康的年龄相关影响。
