Stem Cell Center, 14644Texas Heart Institute, Houston, Texas, USA.
Cardiovascular Pathology, 14644Texas Heart Institute, Houston, Texas, USA.
Cell Transplant. 2021 Jan-Dec;30:9636897211010652. doi: 10.1177/09636897211010652.
Abdominal aortic aneurysms (AAAs) have a high mortality. In small-animal models, multipotent mesenchymal stromal cells (MSCs) have shown benefits in attenuating aneurysm formation. However, an optimal cell delivery strategy is lacking. The NOGA system, which targets cell injections in a less-invasive way, has been used for myocardial cell delivery. Here, we assessed the safety and feasibility of the NOGA system for endovascular delivery of MSCs to the aortic wall in an AAA pig model. We induced AAA in 9 pigs by surgery or catheter induction. MSCs were delivered using the NOGA system 6 or 8 weeks after aneurysm induction. We euthanized the pigs and harvested the aorta for histologic analysis 1, 3, and 7 days after cell delivery. During AAA creation, 1 pig died; 8 pigs completed the study without acute adverse events or complications. The cell delivery procedure was safe and feasible. We successfully injected MSCs directly into the aortic wall in a targeted manner. Histologic and immunohistochemical analyses confirmed transmural injections in the aortic wall area of interest and the presence of MSCs. Our study showed the safety and feasibility of endovascular cell delivery to the aortic wall in a pig model.
腹主动脉瘤（AAA）死亡率高。在小动物模型中，多能间充质基质细胞（MSCs）已显示出在减轻动脉瘤形成方面的益处。然而，缺乏最佳的细胞输送策略。NOGA 系统以微创的方式靶向细胞注射，已用于心肌细胞的输送。在这里，我们评估了 NOGA 系统在 AAA 猪模型中经血管内输送 MSC 到主动脉壁的安全性和可行性。我们通过手术或导管诱导在 9 头猪中诱导 AAA。在动脉瘤诱导后 6 或 8 周，使用 NOGA 系统输送 MSC。在细胞输送后 1、3 和 7 天，我们对猪进行安乐死并采集主动脉进行组织学分析。在 AAA 形成过程中，1 头猪死亡；8 头猪完成了研究，没有发生急性不良事件或并发症。细胞输送程序是安全和可行的。我们成功地以靶向方式将 MSC 直接注入主动脉壁。组织学和免疫组织化学分析证实 MSC 存在于感兴趣的主动脉壁壁内注射部位。我们的研究表明，NOGA 系统在猪模型中经血管内输送细胞到主动脉壁是安全可行的。
