Department of Surgery, Division of Vascular Surgery, Mount Sinai School of Medicine, New York, New York 10029, USA.
J Surg Res. 2011 Sep;170(1):e179-88. doi: 10.1016/j.jss.2011.05.042. Epub 2011 Jul 13.
Cell-based therapies are being evaluated in the setting of degenerative pathophysiologic conditions. The search for the ideal method of delivery and improvement in cell engraftment continue to pose a challenge. This study explores the feasibility of introducing mesenchymal stem cells (MSC) following aortic injury in a porcine model.
Bone marrow-derived MSC were obtained from eight pigs, characterized for the MSC markers CD13 and CD 29, labeled with green fluorescent protein (GFP), and collected for autologous injection in a porcine model of abdominal aortic aneurysm (AAA). The pigs were euthanized (1-7 d) after the procedure to assess the histologic characteristics and presence of MSC in the aortic tissue. Negative controls included noninjured aorta. Tracking of the MSC was conducted by the identification of the GFP-labeled cells using immunofluorescence.
AAA sections stained with hematoxylin and eosin showed disorganization of the aortic tissue; collagen-muscle-elastin stain demonstrated fragmentation of elastin fibers. The presence of the implanted MSC in the aortic wall was evidenced by fluorescent microscopy showing GFP labeled cells. Engraftment of MSC up to 7 d after introduction was observed.
Autologous implantation of bone marrow-derived MSC following aortic injury in a porcine model may be successfully accomplished. The long-term impact and therapeutic value of such cell-based therapy will require further investigation.
细胞疗法正在退行性病理生理条件下进行评估。寻找理想的传递方法和提高细胞移植成功率仍然是一个挑战。本研究探讨了在猪模型中主动脉损伤后引入间充质干细胞（MSC）的可行性。
从 8 头猪中获得骨髓来源的 MSC，用 CD13 和 CD29 等 MSC 标志物进行鉴定，用绿色荧光蛋白（GFP）标记，并收集用于自体注射到猪腹主动脉瘤（AAA）模型中。在手术后 1-7 天处死猪，以评估主动脉组织中的 MSC 的组织学特征和存在情况。阴性对照包括未受伤的主动脉。通过免疫荧光鉴定 GFP 标记的细胞来跟踪 MSC。
用苏木精和伊红染色的 AAA 切片显示主动脉组织的紊乱；胶原-肌肉-弹性蛋白染色显示弹性纤维的碎片。荧光显微镜显示 GFP 标记的细胞表明植入的 MSC 存在于主动脉壁中。在引入后 7 天内观察到 MSC 的植入。
在猪模型中，在主动脉损伤后自体植入骨髓来源的 MSC 可能成功完成。这种基于细胞的治疗的长期影响和治疗价值需要进一步研究。
