Chu Cheng-Feng, Ho Chun-Yee, Tsai Chia-Hsuan, Chen Chien-Tzung, Chen Chih-Hao
Department of Plastic and Reconstructive Surgery, Keelung Chang Gung Memorial Hospital, Keelung 204, Taiwan.
Division of Plastic and Reconstructive Surgery, Department of Surgery, Linkou Chang Gung Memorial Hospital, Taoyuan 333, Taiwan.
Int J Mol Sci. 2025 Apr 30;26(9):4255. doi: 10.3390/ijms26094255.
Cancellous bone grafts are essential in orthopedic and plastic surgeries due to their osteoconductive and osteoinductive properties. However, handling, storage, and preservation challenges impact their viability and effectiveness in bone healing. This study assessed the effects of handling time, storage temperature, and preservation solutions on bone graft viability and osteogenesis using in vitro and in vivo models. Handling times exceeding 10 min significantly reduced cell viability, with 4 °C storage proving superior to 23 °C and 37 °C. In vivo, grafts stored at 4 °C showed enhanced bone regeneration, with PRP-treated grafts demonstrating greater osteogenic potential compared to those stored in blood or PBS. Micro-CT and histological analyses confirmed superior bone volume and tissue integration with PRP, particularly in older grafts. These findings underscore the importance of optimizing perioperative handling protocols. Storage at 4 °C and preservation in PRP emerge as promising strategies for improving bone graft outcomes in clinical applications.
由于具有骨传导性和骨诱导性，松质骨移植在骨科和整形手术中至关重要。然而，处理、储存和保存方面的挑战会影响其在骨愈合中的活力和有效性。本研究使用体外和体内模型评估了处理时间、储存温度和保存溶液对骨移植活力和成骨的影响。处理时间超过10分钟会显著降低细胞活力，4℃储存优于23℃和37℃。在体内，4℃储存的移植物显示出增强的骨再生，与储存在血液或PBS中的移植物相比，经富血小板血浆（PRP）处理的移植物显示出更大的成骨潜力。显微计算机断层扫描（Micro-CT）和组织学分析证实，PRP处理的移植物具有更好的骨体积和组织整合，在较陈旧的移植物中尤为明显。这些发现强调了优化围手术期处理方案的重要性。4℃储存和PRP保存成为改善临床应用中骨移植效果的有前景的策略。
