采用Sebia Capillarys 2 Flex穿刺系统进行血红蛋白分级分离作为评估血红蛋白病的主要方法。

Hemoglobin fractionation by Sebia Capillarys 2 Flex Piercing System as primary method for evaluation of hemoglobinopathies.

作者信息

Poventud-Fuentes Izmarie, Garnett Emily, Vispo Beverly, Elghetany M Tarek, Devaraj Sridevi

机构信息

Department of Pathology and Immunology, Baylor College of Medicine, Houston, TX, United States; Section of Clinical Chemistry, Division of Laboratory Medicine, Department of Pathology, Texas Children's Hospital, Houston, TX, United States.

Section of Clinical Chemistry, Division of Laboratory Medicine, Department of Pathology, Texas Children's Hospital, Houston, TX, United States.

出版信息

Clin Chim Acta. 2021 Aug;519:193-197. doi: 10.1016/j.cca.2021.04.023. Epub 2021 May 1.

DOI:10.1016/j.cca.2021.04.023

PMID:33939955

Abstract

BACKGROUND

Hemoglobin fractionation by capillary zone electrophoresis (CE) is becoming a popular method for the identification of hemoglobin variants that can cause hemoglobinopathies. The goal of this study was to compare the performance of capillary electrophoresis using Sebia Capillarys 2 Flex Piercing system (CE-S) with high-pressure liquid chromatography (HPLC) using Primus Ultra2 Resolution Variants System (HPLC-P) as a primary method in hemoglobinopathy work-up.

METHODS

A total of 306 blood specimens submitted for evaluation of hemoglobinopathies were studied using HPLC-P and CE-S.

RESULTS

The reference ranges for Hb A, A and F agreed well between methods. All common variants containing Hb S and Hb C were detected by both methods. Quantification of Hb A with HPLC-P required a correction in the presence of Hb S, while quantification of Hb A was slightly overestimated by CE-S in the presence of Hb C. Of 41 samples containing other variants, 2 were not identified by HPLC-P and 3 were not identified by CE-S.

CONCLUSION

CE-S provides comparable information to that obtained by HPLC-P and it is a reliable primary method for the evaluation of hemoglobin variants.

摘要

背景

通过毛细管区带电泳（CE）进行血红蛋白分级分离正成为一种用于鉴定可导致血红蛋白病的血红蛋白变体的常用方法。本研究的目的是比较使用赛比毛细管2 Flex穿刺系统（CE-S）的毛细管电泳与使用Primus Ultra2分辨率变体系统（HPLC-P）作为血红蛋白病检查主要方法的高压液相色谱（HPLC）的性能。

方法

使用HPLC-P和CE-S对总共306份提交用于血红蛋白病评估的血液标本进行了研究。

结果

两种方法之间Hb A、A和F的参考范围吻合良好。两种方法均检测到了所有含有Hb S和Hb C的常见变体。在存在Hb S的情况下，使用HPLC-P对Hb A进行定量需要校正，而在存在Hb C的情况下，CE-S对Hb A的定量略有高估。在41份含有其他变体的样本中，有2份未被HPLC-P鉴定出来，3份未被CE-S鉴定出来。